细心根提取物中苯丙素的抗菌活性研究。

IF 2.1 4区 医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Journal of Tropical Medicine Pub Date : 2023-01-01 DOI:10.1155/2023/4983608
Abebe Dagne, Sileshi Degu, Abiy Abebe, Daniel Bisrat
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引用次数: 0

摘要

背景:耐药微生物的出现和迅速蔓延加剧了传染病的治疗,强调了寻找新的、安全的、有效的药物的重要性。在埃塞俄比亚,传统上用细心藤的根来治疗微生物传染病。本研究的目的是评估其根提取物及其主要成分对六种细菌菌株(金黄色葡萄球菌、表皮葡萄球菌、无乳链球菌、大肠杆菌、铜绿假单胞菌和肺炎克雷伯菌)的抗菌活性。方法:采用80%甲醇浸渍法提取风干、粉末状药材。通过硅胶柱层析和CHCl3/MeOH(9:1)重结晶从根提取物中分离得到化合物,并通过ESI-MS和1D-NMR波谱进行了表征。采用琼脂孔扩散法和肉汤微量稀释法测定提取物的抑菌活性。结果:从细鳞草提取物中分离得到一种苯基丙素,并对其进行了表征。该提取物对表皮葡萄球菌的抑菌效果最显著(MIC = 5.33 mg/ml, 200 mg/m时抑菌带直径为24 mm)。紫丁香素对金黄色葡萄球菌(MIC = 13.33 mg/ml)、表皮葡萄球菌(MIC = 16 mg/ml)和无乳葡萄球菌(MIC = 16 mg/ml)均有抑菌活性。尽管测试的最大浓度为16 mg/ml,紫丁香素对革兰氏阴性菌(铜绿假单胞菌、大肠杆菌和肺炎克雷伯菌)没有表现出抗菌活性。结论:总之,研究结果表明紫丁香苷部分参与了根提取物的抗菌活性,从而可能支持该植物的传统药用。
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Antibacterial Activity of a Phenylpropanoid from the Root Extract of Carduus leptacanthus Fresen.

Background: The emergence and rapid spread of antimicrobial drug-resistance microorganisms exacerbate the treatment of infectious diseases, underscoring the importance of finding new, safe, and effective drugs. In Ethiopia, the roots of Carduus leptacanthus have traditionally been employed to treat microbial infectious diseases The aim of this study was to evaluate the antibacterial activity of the root extract and its primary components against six bacterial strains (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumonia).

Methods: The extraction involved maceration of air-dried and powdered roots of C. leptacanthus with 80% methanol. The compound was isolated from the root extract using silica gel column chromatography and recrystallization in CHCl3/MeOH (9 : 1) and was characterized using ESI-MS and 1D-NMR spectroscopy. Antibacterial activity of the extract was assessed using agar well diffusion and broth microdilution methods.

Results: Syringin, a phenylpropanoid, was isolated and characterized from the extract of C. leptacanthus. The extract showed the most substantial efficacy against S. epidermidis (MIC = 5.33 mg/ml and inhibition zone diameter of 24 mm at 200 mg/m). Syringin also elicited antibacterial activity against S. aureus (MIC = 13.33 mg/ml), S. epidermidis (MIC = 16 mg/ml), and S. agalactiae (MIC = 16 mg/ml). Despite being tested up to a maximum concentration of 16 mg/ml, syringin did not exhibit antibacterial activity against the Gram-negative bacteria (P. aeruginosa, E. coli, and K. pneumonia).

Conclusions: In conclusion, the findings suggest that syringin exhibits partial involvement in the root extract's antibacterial activity, thereby potentially supporting the traditional medicinal use of the plant.

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来源期刊
Journal of Tropical Medicine
Journal of Tropical Medicine Immunology and Microbiology-Parasitology
CiteScore
3.90
自引率
4.50%
发文量
0
审稿时长
14 weeks
期刊介绍: Journal of Tropical Medicine is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on all aspects of tropical diseases. Articles on the pathology, diagnosis, and treatment of tropical diseases, parasites and their hosts, epidemiology, and public health issues will be considered. Journal of Tropical Medicine aims to facilitate the communication of advances addressing global health and mortality relating to tropical diseases.
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