α -2巨球蛋白在SARS-CoV-2诱导感染和covid -19后时期的活性

Q3 Biochemistry, Genetics and Molecular Biology Biomeditsinskaya khimiya Pub Date : 2023-09-01 DOI:10.18097/PBMC20236904240
O E Akbasheva, D K Mitrofanova, L V Spirina, Yu G Samoilova, M V Matveeva, D V Podchinenova, O A Oleynik
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引用次数: 0

摘要

通用蛋白酶抑制剂α2-巨球蛋白(α 2 -MG)具有抗病毒和免疫调节活性,被认为是感染过程的重要参与者。α 2 -MG在新型冠状病毒感染和后冠状病毒综合征(long COVID)中的活性尚未研究。我们检查了85例在新型冠状病毒感染SARS-CoV-2的情况下诊断为社区获得性双侧多节段性肺炎的患者。60例患者在冠状病毒感染后5.0±3.6个月进行检查,评估其后病程。在这些患者中,有40人出现并发症,表现为神经学、心脏病学、胃肠病学、皮肤病和支气管肺症状。对照组包括30名条件健康个体,PCR结果为SARS-CoV-2 RNA阴性,缺乏SARS-CoV-2病毒抗体。冠状病毒感染患者血清样品中α 2 -MG活性显著下降,达生理水平的2.5%。同时,与对照组条件健康个体的这些参数相比,α 1 -蛋白酶抑制剂、弹性蛋白酶和胰蛋白酶样蛋白酶的活性分别增加了2.0倍、4.4倍和2.6倍。新冠肺炎后,尽管抑制剂活性趋于正常化,但血清中弹性酶样蛋白酶,特别是胰蛋白酶样蛋白酶的活性仍然升高。在超重个体中,胰蛋白酶样蛋白酶活性的增加最为明显,并与SARS-CoV-2病毒抗体滴度的增加相关。在新冠肺炎后,α 2 -MG活性不仅恢复正常,而且超过对照水平,特别是在有皮肤和神经症状的患者中。有神经症状和皮肤症状的患者α 2 -MG活性分别比无症状者高1.3倍和2.1倍。超重个体在covid后持续低α 2 -MG活性。获得的结果可用于监测covid后时期的进程,并确定并发症的危险群体。
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Alpha-2 macroglobulin activity in SARS-CoV-2 induced infection and in the post-COVID-19 period.

The universal proteinase inhibitor α2-macroglobulin (α₂-MG) exhibiting antiviral and immunomodulatory activities, is considered as an important participant in the infectious process. The activity of α₂-MG in the new coronavirus infection and post-covid syndrome (long COVID) has not been studied yet. We examined 85 patients diagnosed with community-acquired bilateral polysegmental pneumonia developed under conditions of a new coronavirus infection SARS-CoV-2. For assessment of the post-COVID period, 60 patients were examined 5.0±3.6 months after the coronavirus infection. Among these patients, 40 people had complications, manifested in the form of neurological, cardiological, gastroenterological, dermatological, bronchopulmonary symptoms. The control group included 30 conditionally healthy individuals with a negative PCR result for SARS-CoV-2 RNA and lack of antibodies to the SARS-CoV-2 virus. The α₂-MG activity in serum samples of patients with coronavirus infection dramatically decreased, up to 2.5% of the physiological level. This was accompanied by an increase in the activity of the α₁-proteinase inhibitor, elastase- and trypsin-like proteinases by 2.0-, 4.4- and 2.6-fold respectively as compared with these parameters in conditionally healthy individuals of the control. In the post-COVID period, despite the trend towards normalization of the activity of inhibitors, the activity of elastase-like and especially trypsin-like proteinases in serum remained elevated. In overweight individuals, the increase in the activity of trypsin-like proteinases was most pronounced and correlated with an increase in the antibody titer to the SARS-CoV-2 virus. In the post-COVID period, the α₂-MG activity not only normalized, but also exceeded the control level, especially in patients with dermatological and neurological symptoms. In patients with neurological symptoms or with dermatological symptoms, the α₂-MG activity was 1.3 times and 2.1 times higher than in asymptomatic persons. Low α₂-MG activity in the post-COVID period persisted in overweight individuals. The results obtained can be used to monitor the course of the post-COVID period and identify risk groups for complications.

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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
期刊最新文献
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