严格意义上的粒棘球蚴(G1-G3)与罐头棘球蚴(G6/G7)原鞘氨醇的同工酶模式比较

Q2 Biochemistry, Genetics and Molecular Biology Iranian Biomedical Journal Pub Date : 2023-03-01 DOI:10.61186/ibj.3815
Majid Dousti, Seyed Mahmoud Sadjjadi, Rahmat Solgi, Arghavan Vafafar, Yosef Sharifi, Amirhossein Radfar, Gholam Reza Hatam
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摘要

背景:不同基因型的颗粒棘球蚴感染人类和蹄类动物,导致囊性棘球蚴病。伊朗尚未对这种寄生虫的同工酶和分子特征进行过调查。本研究旨在评估伊朗严格意义上的粒细胞棘球蚴病(E. granulosus sensu stricto,s.s.)和卡那氏棘球蚴病(E. canadensis)基因型的同工酶模式:2018年5月至2020年12月,从设拉子、德黑兰、伊拉姆和比尔詹德共分离出32个(8个人和24只动物)囊状棘球蚴病囊肿。提取了 DNA,并通过分子方法确定了其基因型。从包囊中提取酶并进行聚丙烯酰胺凝胶电泳。采用同工酶法检测囊肿样本中葡萄糖-6-磷酸脱氢酶(G6PD)、苹果酸脱氢酶(MDH)、苹果酸酶(ME)、核苷水解酶 1(NH1)和异柠檬酸脱氢酶(ICD)的活性,并与基因分型结果进行比较:结果:样本的DNA序列分析表明,样本中含有75%的E. granulosus s.s.(G1)和25%的E. canadensis(G6)基因型。这两种基因型的 ICD 同工酶模式产生了六条带,相对因子各不相同。两种基因型的 G6PD 也产生了两条相对迁移率不同的条带。MDH 和 NH1 系统显示出两条带的模式,而在 E. granulosus s.s. 基因型中 ME 酶只产生一条带。在 E. canadensis 基因型中,MDH 和 NH1 酶显示一个条带,而 ME 酶显示两个条带:我们的研究结果表明,E. granulosus s.s.和 E. canadensis 基因型的 NH1、G6PD、MDH 和 ME 同工酶模式完全不同。
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Comparison of Isoenzyme Pattern of Echinococcus granulosus sensu stricto (G1-G3) and E. canadensis (G6/G7) Protoscoleces

Background: Different genotypes of Echinococcus granulosus sensu lato (s.l.) infect humans and ungulate animals, causing cystic echinococcosis. Simultaneous isoenzyme, as well as molecular characterizations of this parasite, has not yet been investigated in Iran. The present study aimed to evaluate the isoenzyme pattern of the E. granulosus sensu stricto (s.s.) and E. canadensis genotypes in Iran.

Methods: A total of 32 (8 humans and 24 animals) cystic echinococcosis cysts were isolated from Shiraz, Tehran, Ilam, and Birjand from May 2018 to December 2020. The DNAs were extracted and their genotypes were determined by molecular methods. Enzymes were extracted from the cysts and subjected to polyacrylamide gel electrophoresis. The activities of glucose-6-phosphate sehydrogenase (G6PD), malate dehydrogenase (MDH), malic enzyme (ME), nucleoside hydrolyse 1 (NH1), and isocitrate dehydrogenase (ICD) were examined in the cyst samples using isoenzyme method and compared it with the genotyping findings.

Results: DNA sequence analysis of the samples showed that the specimens contained 75% E. granulosus s.s. (G1) and 25% E. canadensis (G6) genotypes. The isoenzyme pattern of ICD in both genotypes produced a six-band pattern with different relative factors. The G6PD also produced two bands with different relative migrations in both genotypes. The MDH and NH1 systems revealed a two-band pattern, while only one band was generated in the ME enzyme in the E. granulosus s.s. genotype. In the E. canadensis, the MDH and NH1 enzymes showed one band, and the ME enzyme represented a two-band pattern.

Conclusion: Our findings suggest that E. granulosus s.s. and E. canadensis genotypes have entirely different isoenzyme patterns for NH1, G6PD, MDH, and ME.

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来源期刊
Iranian Biomedical Journal
Iranian Biomedical Journal Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.20
自引率
0.00%
发文量
42
审稿时长
8 weeks
期刊最新文献
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