转录因子JAZ亚家族的全基因组分析及BnC08的功能验证。甘蓝型油菜中的JAZ1-1。

Ying Wang, Na Li, Jiepeng Zhan, Xinfa Wang, Xue-Rong Zhou, Jiaqin Shi, Hanzhong Wang
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引用次数: 3

摘要

背景:JAZ亚家族在多种植物的生长发育、胁迫和激素反应中起着重要作用。尽管具有重要意义,但对甘蓝型油菜JAZ亚家族的结构和功能分析仍然有限。结果:与拟南芥中存在的12个JAZ基因(AtJAZ1-AtJAZ12)相比,油菜、甘蓝和甘蓝型油菜的参考基因组中分别存在28个、31个和56个JAZ同源基因,这与油菜科进化过程中已证实的三复制事件一致。系统发育分析表明,来自拟南芥、油菜、甘蓝和甘蓝型油菜的127个JAZ蛋白可分为5个类群。127个JAZs的结构分析表明,这些蛋白具有TIFY和Jas的共同基序,表明它们在芸苔科物种中具有保守性。此外,顺式元件分析表明,主要的基序类型与植物激素、生物和非生物胁迫有关。对甘蓝型油菜具有代表性的11个JAZ基因的qRT-PCR分析表明,不同群体的BnJAZ个体在正常条件下或不同的非生物胁迫和植物激素处理下具有不同的表达模式。其中,BnJAZ52 (BnC08.JAZ1-1)的表达在脱落酸(ABA)、赤霉素(GA)、吲哚乙酸(IAA)、聚乙二醇(PEG)和NaCl处理下均受到显著抑制,而茉莉酸甲酯(MeJA)、低温和涝渍处理下均有诱导作用。表达谱分析显示BnC08。JAZ1-1主要表达于维管束和幼花(包括花瓣、雌蕊、雄蕊和发育中的胚珠)中,而不表达于茎、叶、成熟的花蕊和种子中。亚细胞定位表明该蛋白定位于细胞核,与拟南芥同源物一致。BnC08过表达。JAZ1-1在拟南芥中导致种子重量增加,可能通过调节下游泛素-蛋白酶体途径和磷脂代谢途径的响应基因的表达。结论:bnjjazs亚家族的系统鉴定、系统发育、合成和表达分析有助于我们进一步了解其在甘蓝型油菜逆境和植物激素响应中的作用。此外,对BnC08进行了初步的功能验证。JAZ1-1在拟南芥中的表达表明,该亚家族也可能在调节种子重量中发挥作用。
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Genome-wide analysis of the JAZ subfamily of transcription factors and functional verification of BnC08.JAZ1-1 in Brassica napus.

Background: JAZ subfamily plays crucial roles in growth and development, stress, and hormone responses in various plant species. Despite its importance, the structural and functional analyses of the JAZ subfamily in Brassica napus are still limited.

Results: Comparing to the existence of 12 JAZ genes (AtJAZ1-AtJAZ12) in Arabidopsis, there are 28, 31, and 56 JAZ orthologues in the reference genome of B. rapa, B. oleracea, and B. napus, respectively, in accordance with the proven triplication events during the evolution of Brassicaceae. The phylogenetic analysis showed that 127 JAZ proteins from A. thaliana, B. rapa, B. oleracea, and B. napus could fall into five groups. The structure analysis of all 127 JAZs showed that these proteins have the common motifs of TIFY and Jas, indicating their conservation in Brassicaceae species. In addition, the cis-element analysis showed that the main motif types are related to phytohormones, biotic and abiotic stresses. The qRT-PCR of the representative 11 JAZ genes in B. napus demonstrated that different groups of BnJAZ individuals have distinct patterns of expression under normal conditions or treatments with distinctive abiotic stresses and phytohormones. Especially, the expression of BnJAZ52 (BnC08.JAZ1-1) was significantly repressed by abscisic acid (ABA), gibberellin (GA), indoleacetic acid (IAA), polyethylene glycol (PEG), and NaCl treatments, while induced by methyl jasmonate (MeJA), cold and waterlogging. Expression pattern analysis showed that BnC08.JAZ1-1 was mainly expressed in the vascular bundle and young flower including petal, pistil, stamen, and developing ovule, but not in the stem, leaf, and mature silique and seed. Subcellular localization showed that the protein was localized in the nucleus, in line with its orthologues in Arabidopsis. Overexpression of BnC08.JAZ1-1 in Arabidopsis resulted in enhanced seed weight, likely through regulating the expression of the downstream response genes involved in the ubiquitin-proteasome pathway and phospholipid metabolism pathway.

Conclusions: The systematic identification, phylogenetic, syntenic, and expression analyses of BnJAZs subfamily improve our understanding of their roles in responses to stress and phytohormone in B. napus. In addition, the preliminary functional validation of BnC08.JAZ1-1 in Arabidopsis demonstrated that this subfamily might also play a role in regulating seed weight.

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