环介导等温扩增快速检测东京市区河流ESBL blaSHV基因

IF 0.9 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biocontrol science Pub Date : 2022-01-01 DOI:10.4265/bio.27.209

Masahiko Okai, Rintaro Endo, Masachika Takashio, Masami Ishida, Naoto Urano

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引用次数: 0

摘要

广谱β-内酰胺酶(ESBLs)主要由肠杆菌科革兰氏阴性菌产生。ESBL的主要类型之一是巯基可变(SHV)型ESBL。在此，我们试图建立一种简单快速的检测ESBL blaSHV基因的环介导等温扩增(LAMP)方法。设计的5个引物可以在65℃等温条件下扩增blaSHV基因。采用LF环引物的LAMP方法检测限为1拷贝/管，灵敏度是传统PCR的1万倍。LAMP法还能检测到东京单一河样中blaSHV基因的直接扩增。LAMP方法在医院、水土环境、食品、畜牧等领域具有很大的应用潜力。

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Simple and Rapid Detection of ESBL bla_SHV gene from an Urban River in Tokyo by Loop-Mediated Isothermal Amplification.

Extended-spectrum β-lactamases (ESBLs) are produced mainly by gram-negative bacteria in Enterobacteriaceae. One of the major types of ESBLs is sulfhydryl variable (SHV) -type ESBL. Herein, we attempted to develop a simple and rapid method for the detection of the ESBL blaSHV gene by loop-mediated isothermal amplification (LAMP) . The five-primer set designed could amplify blaSHV gene at an isothermal temperature of 65℃. The detection limit of the LAMP method with the LF loop primer was 1 copy/tube, which was 10,000-fold more sensitive than that of the conventional PCR. The LAMP assay could also detect the direct amplification of blaSHV gene from a single river water sample in Tokyo. The LAMP method has great potential for applications in hospital, soil and water environment, food, and livestock.

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来源期刊

Biocontrol science BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.