罗伊氏乳杆菌l -天冬酰胺酶利用Caspase-8和Caspase-9诱导乳腺癌细胞凋亡。

Breast disease Pub Date : 2023-01-01 DOI:10.3233/BD-220074
Zaman Hussein Hassan, Ibtesam Ghadban Auda, Likaa Hamied Mahdi
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引用次数: 0

摘要

背景:乳腺癌是全世界女性中最致命的癌症类型。许多化疗药物针对乳腺癌,然而,它们有可怕的副作用。控制癌细胞生长的一种方法是靶向凋亡。目的:通过抑制Caspases 8和Caspases 9,从人乳罗伊氏乳杆菌分离物中纯化l -天冬酰胺酶,诱导乳腺癌细胞凋亡。方法:从人母乳中鉴定出产l -天冬酰胺酶活性最高的罗伊氏乳杆菌分离株,并进行l -天冬酰胺酶纯化。MTT细胞活力测定法用于测定酶的毒性。利用乳腺癌细胞系研究了该酶对caspase 8和caspase 9基因表达的影响。结果:MTT细胞活力测定显示,当酶的浓度为3.125、6.25、12.5、25、50、100 μg/ml, IC50为4.305 μg/ml时,细胞死亡的抑制率在30% ~ 80%之间。用IC50浓度的酶处理乳腺细胞系。在IC50浓度下,l -天冬酰胺处理的乳腺癌细胞株中Cas8和Cas9基因表达上调(表达倍数分别为2.071和1.197)。结论:母乳罗伊氏乳杆菌l -天冬酰胺酶通过上调Cas8和Cas9诱导乳腺癌细胞凋亡。罗伊氏乳杆菌l -天冬酰胺酶治疗可能是治疗乳腺癌的有希望的方法。此外,该结果可能强调了这样一个事实,即人类母乳中产生l -天冬酰胺酶的罗伊氏乳杆菌分离物可能有助于改善甚至预防乳腺癌。
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L-asparaginase from human breast milk Lactobacillus reuteri induces apoptosis using therapeutic targets Caspase-8 and Caspase-9 in breast cancer cell line.

Background: Breast cancer is the most fatal type of cancer in women worldwide. Many chemotherapeutics targeted breast cancer however, they have frightening side effects. One method of controlling cancer cell growth is targeting apoptosis.

Objective: This study aimed to induce apoptosis in breast cancer cells by purifying L-asparaginase from human breast milk Lactobacillus reuteri isolates via inhibition of Caspases 8 and 9.

Methods: The best L. reuteri isolates producing L-asparagine with the highest enzyme activity were identified from human breast milk and chosen for L-asparaginase purification. The MTT cell viability assay used for measure the toxicity of the enzyme. Breast cancer cell line was used to study the effect of the enzyme on the caspase 8 and caspase 9 gene expression.

Results: The MTT cell viability assay showed the inhibition rates ranged between 30% and 80%, of cell death, occurred when 3.125, 6.25, 12.5, 25, 50, and 100 μg/ml of the enzyme used and IC50 was 4.305 μg/ml. The breast cell lines were treated with the enzyme at a concentration of IC50 value. The Cas8 and Cas9 genes expression in L-asparagine treated breast cancer cell line at a concentration of IC50 value were upregulated (the fold of gene expression are 2.071 and 1.197 respectively).

Conclusions: Breast milk L. reuteri L-asparaginase induces apoptosis via Cas8 and Cas9 upregulation in the breast cancer cell line. L. reuteri L-asparaginase treatment may be the hopeful approach for the management of breast cancer. Furthermore, the results may highlight the fact that the presence of L-asparaginase-producing L. reuteri isolates in human breast milk may aid in breast cancer improvement or even prevention.

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来源期刊
Breast disease
Breast disease Medicine-Oncology
CiteScore
1.80
自引率
0.00%
发文量
59
期刊介绍: The recent expansion of work in the field of breast cancer inevitably will hasten discoveries that will have impact on patient outcome. The breadth of this research that spans basic science, clinical medicine, epidemiology, and public policy poses difficulties for investigators. Not only is it necessary to be facile in comprehending ideas from many disciplines, but also important to understand the public implications of these discoveries. Breast Disease publishes review issues devoted to an in-depth analysis of the scientific and public implications of recent research on a specific problem in breast cancer. Thus, the reviews will not only discuss recent discoveries but will also reflect on their impact in breast cancer research or clinical management.
期刊最新文献
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