BNYVV和BSCTV联合感染对转基因甜菜植株抗根病能力的评价。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-10-01 Epub Date: 2023-09-01 DOI:10.1007/s11248-023-00364-8
Maryam Khoshnami, Bahar Zare, Hamideh Mardani-Mehrabad, Farshad Rakhshandehroo, Mohammad Amin Baghery, Mohammad Ali Malboobi
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引用次数: 0

摘要

甜菜是一种重要的经济作物,也是世界蔗糖的主要来源之一。甜菜坏死黄脉病毒(BNYVV)和甜菜严重卷顶病毒(BSCTV)是甜菜中两种广泛存在的病毒,对其性能造成严重损害。此前,我们通过在有义和反义方向引入携带编码DNA序列CP21的病毒外壳蛋白的构建体,在甜菜中成功地产生了基于RNA沉默的对BNYVV的抗性。然而,作为协同作用的一部分,RNA沉默介导的对特定病毒的耐药性可能会受到其他病毒的影响。在本研究中,我们在两组转基因事件中测定了诱导的对BNYVV的抗性的特异性,S3和S6分别携带带有或不带有CP21编码序列的5'-UTR。这些事件受到BNYVV、BSCTV的伊朗分离物(BSCTV Ir)或两者的病毒挑战。所有只接种BSCTV-Ir的植物都表现出卷曲的叶子症状。然而,部分耐药性在S3事件中是明显的,如轻微症状和BSCTV Ir外壳蛋白编码序列的PCR扩增减少所示。基于所提供的数据,除了S3-229这一事件外,几乎所有与BSCTV-Ir共感染的转基因植物对BNYVV的抗性都是稳定的。总的来说,共感染似乎不会影响转基因植物对BNYVV的抗性。这些发现表明,在转基因甜菜中引入的RNA沉默介导的对BNYVV的抗性是特异性的,并且在与异源病毒共同感染后不会被抑制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Assessment of co-infection with BNYVV and BSCTV on resistance against Rhizomania disease in transgenic sugar beet plants.

Sugar beet is an economically important crop and one of the major sources of sucrose around the world. Beet necrotic yellow vein virus (BNYVV) and Beet severe curly top virus (BSCTV) are two widespread viruses in sugar beet that cause severe damage to its performance. Previously, we have successfully produced resistance to BNYVV based on RNA silencing in sugar beet by introducing constructs carrying the viral coat-protein-encoding DNA sequence, CP21, in sense and anti-sense orientations. Yet, the RNA silencing-mediated resistance to a specific virus could be affected by other ones as a part of synergistic interactions. In this study, we assayed the specificity of the induced resistance against BNYVV in two sets of transgenic events, S3 and S6 carrying 5'-UTR with or without CP21-coding sequences, respectively. These events were subjected to viral challenges with either BNYVV, an Iranian isolate of BSCTV (BSCTV-Ir) or both. All the plants inoculated with just BSCTV-Ir displayed curly-leaf symptoms. However, partial resistance was evident in S3 events as shown by mild symptoms and reduced PCR amplification of the BSCTV-Ir coat protein encoding sequence. Based on the presented data, resistance to BNYVV was stable in almost all the transgenic plants co-infected with BSCTV-Ir, except for one event, S3-229. In general, it seems that the co-infection does not affect the resistance to BNYVV in transgenic plants. These findings demonstrated that the introduced RNA silencing-mediated resistance against BNYVV in transgenic sugar beets is specific and is not suppressed after co-infection with a heterologous virus.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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