利用 Enrich TROVO 平台选择性扩增靶细胞。

IF 2.2 4区 工程技术 Q3 BIOCHEMICAL RESEARCH METHODS BioTechniques Pub Date : 2023-08-01 Epub Date: 2023-08-08 DOI:10.2144/btn-2023-0038
Prem Shrestha, Stephen Rotatori, Kirby Madden-Hennessey, Christina Mohammed, Chi-Han Yang, Jordan Urbani, Joseph Pettinelli, Xueqi Liu, Qi Zhao
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引用次数: 0

摘要

从不同的细胞群中富集目标细胞克隆对许多生命科学应用至关重要。我们开发了一种新方法,利用 Enrich TroVo 系统(Enrich Biosystems Inc.)该系统利用微加工和光学技术的优势,通过使用小型水凝胶孔来分离所需的细胞群,并采用创新的贴片技术来选择性地消除不需要的细胞。这种方法可以分离和生长所需的细胞,并将对细胞活力和增殖的影响降至最低。我们利用两种模型细胞成功分离并扩增了所需细胞的克隆细胞群。与荧光激活细胞分拣法相比,Enrich TroVo 系统为分离敏感的粘附细胞(即病人衍生细胞)提供了一种很有前途的替代方法。
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Selective expansion of target cells using the Enrich TROVO platform.

Enriching target cell clones from diverse cell populations is vital for many life science applications. We have developed a novel method to rapidly and efficiently purify specific clonal cell populations from a larger, heterogeneous group using the Enrich TroVo system (Enrich Biosystems Inc., CT, USA). This system takes advantage of microfabrication and optical technologies by utilizing small hydrogel wells to separate desired cell populations and an innovative patching technique to selectively eliminate undesired cells. This method allows the isolation and growth of desired cells with minimal impact on their viability and proliferation. We successfully isolated and expanded clonal cell populations of desired cells using two model cells. Compared with fluorescence-activated cell sorting, Enrich TroVo system offers a promising alternative for isolating of sensitive, adherent cells, that is, patient-derived cells.

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来源期刊
BioTechniques
BioTechniques 工程技术-生化研究方法
CiteScore
4.40
自引率
0.00%
发文量
68
审稿时长
3.3 months
期刊介绍: BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies. Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers: Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices Surveys of technical approaches related to broad fields of research Reviews discussing advancements in techniques and methods related to broad fields of research Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.
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