菌丝渗出物的代谢组学分析提供了蜜环菌拮抗真菌的见解。

IF 4.6 2区 生物学 Q1 MYCOLOGY Mycology Pub Date : 2023-01-01 DOI:10.1080/21501203.2023.2238753
Jian Zhan, Jing Yuan, Jianwei Liu, Fengming Zhang, Fuqiang Yu, Yanliang Wang
{"title":"菌丝渗出物的代谢组学分析提供了蜜环菌拮抗真菌的见解。","authors":"Jian Zhan,&nbsp;Jing Yuan,&nbsp;Jianwei Liu,&nbsp;Fengming Zhang,&nbsp;Fuqiang Yu,&nbsp;Yanliang Wang","doi":"10.1080/21501203.2023.2238753","DOIUrl":null,"url":null,"abstract":"<p><p>The genus <i>Armillaria</i> has high edible and medical values, with zones of antagonism often occurring when different species are paired in culture on agar media, while the antagonism-induced metabolic alteration remains unclear. Here, the metabolome of mycelial exudates of two Chinese <i>Armillaria</i> biological species, C and G, co-cultured or cultured separately was analysed to discover the candidate biomarkers and the key metabolic pathways involved in <i>Armillaria</i> antagonists. A total of 2,377 metabolites were identified, mainly organic acids and derivatives, lipids and lipid-like molecules, and organoheterocyclic compounds. There were 248 and 142 differentially expressed metabolites between group C-G and C, C-G, and G, respectively, and fourteen common differentially expressed metabolites including malate, uracil, Leu-Gln-Arg, etc. Metabolic pathways like TCA cycle and pyrimidine metabolism were significantly affected by C-G co-culture. Additionally, 156 new metabolites (largely organic acids and derivatives) including 32 potential antifungal compounds, primarily enriched into biosynthesis of secondary metabolites pathways were identified in C-G co-culture mode. We concluded that malate and uracil could be used as the candidate biomarkers, and TCA cycle and pyrimidine metabolism were the key metabolic pathways involved in <i>Armillaria</i> antagonists. The metabolic changes revealed in this study provide insights into the mechanisms underlying fungal antagonists.</p>","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"14 3","pages":"264-274"},"PeriodicalIF":4.6000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/02/ff/TMYC_14_2238753.PMC10424624.pdf","citationCount":"0","resultStr":"{\"title\":\"Metabolomics analysis of mycelial exudates provides insights into fungal antagonists of <i>Armillaria</i>.\",\"authors\":\"Jian Zhan,&nbsp;Jing Yuan,&nbsp;Jianwei Liu,&nbsp;Fengming Zhang,&nbsp;Fuqiang Yu,&nbsp;Yanliang Wang\",\"doi\":\"10.1080/21501203.2023.2238753\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The genus <i>Armillaria</i> has high edible and medical values, with zones of antagonism often occurring when different species are paired in culture on agar media, while the antagonism-induced metabolic alteration remains unclear. Here, the metabolome of mycelial exudates of two Chinese <i>Armillaria</i> biological species, C and G, co-cultured or cultured separately was analysed to discover the candidate biomarkers and the key metabolic pathways involved in <i>Armillaria</i> antagonists. A total of 2,377 metabolites were identified, mainly organic acids and derivatives, lipids and lipid-like molecules, and organoheterocyclic compounds. There were 248 and 142 differentially expressed metabolites between group C-G and C, C-G, and G, respectively, and fourteen common differentially expressed metabolites including malate, uracil, Leu-Gln-Arg, etc. Metabolic pathways like TCA cycle and pyrimidine metabolism were significantly affected by C-G co-culture. Additionally, 156 new metabolites (largely organic acids and derivatives) including 32 potential antifungal compounds, primarily enriched into biosynthesis of secondary metabolites pathways were identified in C-G co-culture mode. We concluded that malate and uracil could be used as the candidate biomarkers, and TCA cycle and pyrimidine metabolism were the key metabolic pathways involved in <i>Armillaria</i> antagonists. The metabolic changes revealed in this study provide insights into the mechanisms underlying fungal antagonists.</p>\",\"PeriodicalId\":18833,\"journal\":{\"name\":\"Mycology\",\"volume\":\"14 3\",\"pages\":\"264-274\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/02/ff/TMYC_14_2238753.PMC10424624.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/21501203.2023.2238753\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/21501203.2023.2238753","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

蜜环菌属具有很高的食用和药用价值,当不同物种在琼脂培养基上配对培养时,经常出现拮抗区,而拮抗诱导的代谢改变尚不清楚。本研究分析了中国两种蜜环菌种C和G共培养或单独培养的菌丝渗出物的代谢组学,以发现蜜环菌拮抗剂的候选生物标志物和关键代谢途径。共鉴定出2377种代谢物,主要是有机酸及其衍生物、脂质和类脂质分子以及有机杂环化合物。C-G组与C组、C-G组和G组差异表达代谢物分别为248种和142种,共有14种差异表达代谢物,包括苹果酸盐、尿嘧啶、Leu-Gln-Arg等。C-G共培养显著影响了TCA循环和嘧啶代谢等代谢途径。此外,在C-G共培养模式下,鉴定出156种新的代谢物(主要是有机酸及其衍生物),包括32种潜在的抗真菌化合物,主要富集到次生代谢物的生物合成途径中。我们认为苹果酸和尿嘧啶可以作为候选生物标志物,TCA循环和嘧啶代谢是蜜环菌拮抗剂的关键代谢途径。本研究揭示的代谢变化为真菌拮抗剂的机制提供了见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Metabolomics analysis of mycelial exudates provides insights into fungal antagonists of Armillaria.

The genus Armillaria has high edible and medical values, with zones of antagonism often occurring when different species are paired in culture on agar media, while the antagonism-induced metabolic alteration remains unclear. Here, the metabolome of mycelial exudates of two Chinese Armillaria biological species, C and G, co-cultured or cultured separately was analysed to discover the candidate biomarkers and the key metabolic pathways involved in Armillaria antagonists. A total of 2,377 metabolites were identified, mainly organic acids and derivatives, lipids and lipid-like molecules, and organoheterocyclic compounds. There were 248 and 142 differentially expressed metabolites between group C-G and C, C-G, and G, respectively, and fourteen common differentially expressed metabolites including malate, uracil, Leu-Gln-Arg, etc. Metabolic pathways like TCA cycle and pyrimidine metabolism were significantly affected by C-G co-culture. Additionally, 156 new metabolites (largely organic acids and derivatives) including 32 potential antifungal compounds, primarily enriched into biosynthesis of secondary metabolites pathways were identified in C-G co-culture mode. We concluded that malate and uracil could be used as the candidate biomarkers, and TCA cycle and pyrimidine metabolism were the key metabolic pathways involved in Armillaria antagonists. The metabolic changes revealed in this study provide insights into the mechanisms underlying fungal antagonists.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Mycology
Mycology Medicine-Infectious Diseases
CiteScore
9.10
自引率
0.00%
发文量
18
审稿时长
13 weeks
期刊最新文献
Isolation of triazole-resistant Aspergillus fumigatus harbouring cyp51A mutations from five patients with invasive pulmonary aspergillosis in Yunnan, China Analytical and clinical validation of multiplex droplet digital PCR assay for detecting pathogenic fungal infection in lungs Optimisation of hypocrellin production in Shiraia -like fungi via genetic modification involving a transcription factor gene and a putative monooxygenase gene Call me by your name: Considerations of DNA sequences as types within wider discussions on fungal nomenclature A comprehensive review of secondary metabolites from the genus Agrocybe : Biological activities and pharmacological implications
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1