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引用次数: 1
摘要
溶解多糖单氧化酶（LPMOs）是一种铜金属酶，可氧化裂解多糖，在病原体生物学、碳循环和生物技术领域具有重要作用。由于 LPMOs 对较小的可溶性糖底物具有活性，因此可以通过高分辨率的 X 射线晶体学对其与多种底物的相互作用进行详细的结构表征，因此人们将 LPMOs 家族 AA9 同工酶 A（LsAA9_A）作为一个模型系统进行了广泛的研究。本文介绍了无碳水化合物的 LsAA9_A 在铜（II）静止态时的 X 射线/中子联合室温晶体结构，该结构是根据 X 射线和中子数据分别以 2.1 和 2.8 Å 的分辨率精制而成的。研究结果通过实验确定了 LsAA9_A 中铜（II）配位残基和第二壳残基的质子态，为今后对 LPMO 碳水化合物复合物进行中子晶体学研究铺平了道路。
Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature.
Lytic polysaccharide monooxygenases (LPMOs) are copper metalloenzymes which cleave polysaccharides oxidatively and are important in pathogen biology, carbon cycling and biotechnology. The Lentinus similis family AA9 isoform A (LsAA9_A) has been extensively studied as a model system because its activity towards smaller soluble saccharide substrates has allowed detailed structural characterization of its interaction with a variety of substrates by X-ray crystallography at high resolution. Here, the joint X-ray/neutron room-temperature crystallographic structure of carbohydrate-free LsAA9_A in the copper(II) resting state refined against X-ray and neutron data at 2.1 and 2.8 Å resolution, respectively, is presented. The results provide an experimental determination of the protonation states of the copper(II)-coordinating residues and second-shell residues in LsAA9_A, paving the way for future neutron crystallographic studies of LPMO-carbohydrate complexes.
期刊介绍：
Acta Crystallographica Section F is a rapid structural biology communications journal.
Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal.
The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles.
Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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