大肠杆菌/志贺氏菌泛基因组中四硫酸还原酶操纵子的出现

IF 3.9 3区 生物学 Q2 MICROBIOLOGY MicrobiologyOpen Pub Date : 2022-11-06 DOI:10.1002/mbo3.1333
Floyd G. Adsit Jr, Thomas A. Randall, Jacqueline Locklear, David M. Kurtz
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引用次数: 0

摘要

大肠杆菌致病性变异(病原菌)通常具有明确的毒力特征,并且由于大肠杆菌基因组的相当大的可塑性,易受杂交身份进化的影响。我们已经从用于研究动物的纯化饮食中分离出一株菌株,这进一步证明了大肠杆菌获得新遗传元件的能力,可能导致新病原体的出现。利用下一代测序获得全基因组图谱,我们报道了一株非典型大肠杆菌EcoFA807-17,该菌株具有四硫酸盐还原酶(ttr)操纵子,该操纵子能够利用四硫酸盐作为电子受体,从而促进哺乳动物肠道等厌氧环境中的呼吸。ttr操纵子是几种肠道病原体的有效毒力因子,最突出的是肠沙门氏菌。然而,在野生型大肠杆菌或志贺氏菌中,染色体整合四硫酸还原酶基因的存在似乎并没有先前的报道。因此,这种毒力因子的出现可能标志着大肠杆菌和志贺氏菌致病性新机制的进化,并可能潜在地改变现有使用四硫酸还原酶作为检测肠沙门氏菌的独特标记物的检测方法的有效性。
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The emergence of the tetrathionate reductase operon in the Escherichia coli/Shigella pan-genome

Escherichia coli pathogenic variants (pathovars) are generally characterized by defined virulence traits and are susceptible to the evolution of hybridized identities due to the considerable plasticity of the E. coli genome. We have isolated a strain from a purified diet intended for research animals that further demonstrates the ability of E. coli to acquire novel genetic elements leading potentially to emergent new pathovars. Utilizing next generation sequencing to obtain a whole genome profile, we report an atypical strain of E. coli, EcoFA807-17, possessing a tetrathionate reductase (ttr) operon, which enables the utilization of tetrathionate as an electron acceptor, thus facilitating respiration in anaerobic environments such as the mammalian gut. The ttr operon is a potent virulence factor for several enteric pathogens, most prominently Salmonella enterica. However, the presence of chromosomally integrated tetrathionate reductase genes does not appear to have been previously reported in wild-type E. coli or Shigella. Accordingly, it is possible that the appearance of this virulence factor may signal the evolution of new mechanisms of pathogenicity in E. coli and Shigella and may potentially alter the effectiveness of existing assays using tetrathionate reductase as a unique marker for the detection of Salmonella enterica.

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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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