低频脉冲电磁场对胶质母细胞瘤细胞氧化应激的细胞保护作用。

IF 1.3 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY General physiology and biophysics Pub Date : 2023-01-01 DOI:10.4149/gpb_2022056
Çiğdem Gökçek-Saraç, Tuğçe Şimşek, Serdar Karakurt
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引用次数: 0

摘要

低频脉冲电磁场(PEMF)可能具有抗氧化应激破坏作用的细胞保护作用。目的是研究短期低频PEMF在高剂量过氧化氢(H2O2)治疗后对胶质母细胞瘤细胞系是否具有细胞保护作用。U87-MG细胞分为4组:假对照组;PEMF组(暴露于PEMF的细胞);H2O2组(细胞分别在30 min和48 h的时间间隔中被H2O2处理);H2O2+PEMF组(细胞在H2O2处理后分别在30min和48h时间间隔暴露于PEMF)。测定细胞活力、活性氧水平、谷胱甘肽过氧化物酶活性和谷胱甘肽含量。PEMF对不同H2O2处理时间间隔引发的氧化应激的细胞保护作用可能是通过提高细胞活力、提高抗氧化酶活性/量和降低活性氧水平来调节的。此外,PEMF的细胞保护作用随H2O2处理时间间隔的不同而不同。鉴于这些发现,需要进一步在体内和/或体外研究pemf的神经生理作用及其潜在的分子机制,以阐明抗氧化防御机制的神经毒性或神经保护作用。
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Cytoprotective effects of low-frequency pulsed electromagnetic field against oxidative stress in glioblastoma cells.

The low-frequency pulsed electromagnetic field (PEMF) may have possible cytoprotective effects against the destructive effects of oxidative stress. The goal was to investigate if shortterm low-frequency PEMF has cytoprotective effects in glioblastoma cell line following high-dose hydrogen peroxide (H2O2) treatment. U87-MG cells were divided into four groups: Sham-control group; PEMF group (cells exposed to PEMF); H2O2 group (cells treated with H2O2 at time intervals 30 min and 48 h, respectively); H2O2+PEMF group (cells exposed to PEMF after H2O2 treatment at time intervals 30 min and 48 h, respectively). The cell viability, levels of reactive oxygen species, glutathione peroxidase activity, and the amount of glutathione were measured. The cytoprotective effect of PEMF against deleterious effects of oxidative stress triggered by different time interval of H2O2 treatment might be mediated by the increase in the cell viability, the elevation in the antioxidant enzyme activity/amount, and the decrease in the reactive oxygen species level. In addition, the cytoprotective effect of PEMF varies depending on different time intervals of H2O2 treatment. In the light of these findings, further in vivo and/or in vitro studies on neurophysiological effects of PEMFs and their underlying molecular mechanisms are needed to elucidate neurotoxic or neuroprotective role against antioxidant defense mechanisms.

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来源期刊
General physiology and biophysics
General physiology and biophysics 生物-生化与分子生物学
CiteScore
2.70
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: General Physiology and Biophysics is devoted to the publication of original research papers concerned with general physiology, biophysics and biochemistry at the cellular and molecular level and is published quarterly by the Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences.
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