斑胸草雀(Taeniopygia guttata)群体中多瘤病毒相关的播散性t细胞淋巴瘤

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES Comparative medicine Pub Date : 2023-09-11 DOI:10.30802/AALAS-CM-23-000011
Katherine A Shuster, Tzushan S Yang, Kate T Snyder, Nicole Creanza, Patrick K Mitchell, Laura B Goodman, Jennifer K Grenier, Nicholas M Tataryn, Lauren E Himmel, Katherine N Gibson-Corley
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引用次数: 0

摘要

在一个封闭的研究群体中,四只斑胸草雀表现出不同的临床症状,包括肿块、皮肤病变、颤抖和/或羽毛褶皱。这些鸟对治疗没有反应;其中3人死亡,1人被安乐死。所有4例均提交尸检以确定临床症状的原因。鸟类的大体尸检和组织病理学检查结果显示为多器官圆细胞瘤变,包括皮肤、肝脏、肾脏和生殖道,肿瘤细胞内有核内包涵体。在所有4例病例中,免疫组织化学染色显示70%至80%的肿瘤圆形细胞对CD3具有强免疫反应性,相对较小的亚群对Pax5具有免疫阳性。这些结果支持t细胞淋巴瘤的诊断。一个病例的冷冻肝组织被提交给下一代测序(NGS),结果显示病毒RNA与最初在欧洲金翅雀中发现的金丝雀多瘤病毒34639株具有100%的序列同源性。另一病例经福尔马林固定石蜡包埋的病毒卷也提交了NGS,结果显示该病毒RNA序列与最初在金丝雀中鉴定的金丝雀多瘤病毒株37273有97.2%的同源性。为了原位定位病毒,在冷冻肝组织中使用设计用于靶向测序病毒VP1基因的探针进行RNAscope杂交。在所有4例病例中,肿瘤细胞中均检测到播散性和鲁棒性杂交信号。这些发现表明,多瘤病毒在斑胸草雀中具有致癌的潜力。
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Polyomavirus-associated Disseminated T-cell Lymphoma in a Colony of Zebra Finches (Taeniopygia guttata).

Four zebra finches in a closed research colony presented with variable clinical signs, including masses, skin lesions,shivering, and/or ruffled feathers. These birds were not responsive to treatment efforts; 3 died and one was euthanized. All4 were submitted for necropsy to determine the cause of the clinical signs. Gross necropsy and histopathologic findings fromall birds resulted in a diagnosis of round cell neoplasia in multiple organs, including the skin, liver, kidney, and reproductivetract, with intranuclear inclusion bodies in the neoplastic cells. In all 4 cases, immunohistochemical staining showed strongimmunoreactivity for CD3 in 70% to 80% of the neoplastic round cells, with a relatively small subset that were immunopositivefor Pax5. These findings supported a diagnosis of T-cell lymphoma. Frozen liver tissue from one case was submittedfor next-generation sequencing (NGS), which revealed viral RNA with 100% sequence homology to canary polyomavirusstrain 34639 that had originally been identified in a European goldfinch. Formalin-fixed paraffin-embedded scrolls fromanother case were also submitted for NGS, which revealed viral RNA with 97.2% sequence homology to canary polyomavirusstrain 37273 that had originally been identified in a canary. To localize the virus in situ, RNAscope hybridizationwas performed using a probe designed to target the VP1 gene of the sequenced virus in frozen liver tissue. In all 4 cases,disseminated and robust hybridization signals were detected in neoplastic cells. These findings indicate that polyomaviruseshave the potential to be oncogenic in zebra finches.

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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
期刊最新文献
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