Solomon Blinchevsky, Aparna Ramasubramanian, Douglas Borchman, Shanzeh Sayied, Krithika Venkatasubramanian
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引用次数: 0
摘要
目的:帕金森病(PD)患者表现出不稳定的泪膜。泪膜脂质组成和结构与泪膜稳定性和干眼有关，PD患者的泪膜脂质尚未被表征。本研究的目的是利用1H-NMR和红外光谱来表征PD供体的Meibum泪液脂质。方法:比较三个队列:PD供者的细胞代谢(Mp) n = 10, PD合并干眼供者的细胞代谢(Mpd) n = 3，非PD供者的细胞代谢(Mn) n = 29。结果:Mp与Mn在烃类分支上无显著差异(P > 0.05)。Mn的胆固醇酯含量是Mp的2倍，P < 0.0001。与Mn相比，Mp的相变协同性显著降低37%，P < 0.0001。与Mp和Mn相比，Mpd更有序(更硬)，P < 0.0001。结论:脂质组成和结构的变化可能是PD患者干眼症易感性增加的标志。与Mn相比，Mp的合作相变较少，这表明Mp比Mn更具异质性和/或含有更多的污染物。这些数据支持这样一种观点，即更多有序的脂质会导致干眼症。
Meibum Lipid Composition and Conformation in Parkinsonism.
Purpose: Patients with Parkinson's disease (PD) exhibit unstable tear films. Tear film lipid composition and structure are related to tear film stability and dry eye and tear lipids have not been characterized in people with PD. The aim of this study is to characterize Meibum tear lipids in donors with PD using 1H-NMR and infrared spectroscopy.
Methods: Three cohorts were compared: meibum from donors with PD (Mp) n = 10, meibum from donors with PD and dry eye (Mpd) n = 3, meibum from donors without PD (Mn) n = 29.
Results: There were no significant differences, P > 0.05, in hydrocarbon branching for Mp compared with Mn. Mn contained twice as much cholesteryl esters compared with Mp, P < 0.0001. The cooperativity of the phase transition was significantly 37% lower for Mp compared with Mn, P < 0.0001. Mpd was much more ordered (stiffer) with compared with Mp and Mn, P < 0.0001.
Conclusion: Changes in meibum lipid composition and structure could be a marker for and/or contribute to increase the susceptibility of dry eye in patients with PD. A less cooperative phase transition for Mp compared with Mn indicates that Mp was more heterogeneous and/or contained more contaminants than Mn. The data support the idea that more ordered lipid contributes to dry eye.
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