CXCL12/SDF-1在人骨髓基质细胞中差异表达mirna的表征

Q2 Biochemistry, Genetics and Molecular Biology Biomolecular Concepts Pub Date : 2021-10-13 DOI:10.1515/bmc-2021-0015

Matthew L Potter, Kathryn Smith, Sagar Vyavahare, Sandeep Kumar, Sudharsan Periyasamy-Thandavan, Mark Hamrick, Carlos M Isales, William D Hill, Sadanand Fulzele

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引用次数: 4

摘要

基质细胞衍生因子1 (SDF-1)影响骨髓基质细胞(BMSC)迁移、成骨分化和骨折愈合。我们假设SDF-1通过表观遗传调控，特别是通过microrna (miRNAs)介导其对骨髓间充质干细胞的一些影响。mirna是针对特定mRNA并阻止其翻译的小非编码rna。我们进行了全球miRNA分析，并确定了几种miRNA在SDF-1治疗后的差异表达。基因表达综合(GEO)数据集分析显示，这些mirna在成骨分化和骨折愈合中发挥重要作用。KEGG和GO分析表明，SDF-1依赖性mirna的变化影响多种细胞途径，包括脂肪酸生物合成、甲状腺激素信号传导和粘蛋白型o -聚糖生物合成途径。此外，生物信息学分析显示，一些mirna靶基因与干细胞迁移和分化有关。本研究结果表明，SDF-1通过miRNA调控诱导其对骨髓间充质干细胞功能的部分影响。

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Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells.

Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.

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来源期刊

Biomolecular Concepts Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

5.30

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： BioMolecular Concepts is a peer-reviewed open access journal fostering the integration of different fields of biomolecular research. The journal aims to provide expert summaries from prominent researchers, and conclusive extensions of research data leading to new and original, testable hypotheses. Aspects of research that can promote related fields, and lead to novel insight into biological mechanisms or potential medical applications are of special interest. Original research articles reporting new data of broad significance are also welcome. Topics: -cellular and molecular biology- genetics and epigenetics- biochemistry- structural biology- neurosciences- developmental biology- molecular medicine- pharmacology- microbiology- plant biology and biotechnology.