抗rage(受体晚期糖基化终产物)抗体通过降糖和抗炎机制改善大鼠糖尿病视网膜病变。

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2022-10-01 DOI:10.52547/rbmb.11.3.394
Ramzi Amin, Tiara Bunga Indiarsih, Prima Maya Sari, Petty Purwanita
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引用次数: 1

摘要

背景:受体晚期糖基化终产物(RAGE)的激活在糖尿病视网膜病变(DR)的进展中起着至关重要的作用。本研究旨在探讨抗RAGE抗体(RAGE拮抗剂)通过其降血糖和抗炎机制在糖尿病视网膜病变大鼠中抑制DR进展的作用。方法:将30只雄性Wistar大鼠随机分为5组。实验组分为正常对照组、未经治疗的DR组、抗rage DR组(1 ηg/kg BW、10 ηg/kg BW、100 ηg/kg BW)。为了评估糖尿病视网膜病变,每周使用16倍放大镜放置在大鼠眼睛前拍摄眼底照片。葡萄糖氧化酶-过氧化物酶法测定血糖。采用酶联免疫吸附测定(ELISA)试剂盒检测视网膜TNF-α和VEGF水平。结果:本研究结果显示,抗rage治疗剂量为10和100 ηg/kg BW时,HbA1c水平显著高于正常对照组(p< 0.05),显著低于糖尿病组(p< 0.05)。DR+抗rage 10和100 ηg/kg BW组的平均血管直径显著低于糖尿病视网膜病变组(p< 0.05)。与DR组相比,抗rage 10和100 ηg/kg BW组能显著降低VEGF水平(p< 0.05)。讨论:本研究显示,在10和100 ηg/kg BW剂量下,抗rage抗体通过降糖作用和抗炎机制改善Wistar大鼠糖尿病视网膜病变。
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Anti-RAGE (Receptor Advanced Glycation End products) Antibody Improves Diabetic Retinopathy in Rats via Hypoglycemic and Anti-inflammatory Mechanism.

Background: Receptor advanced glycation end products (RAGE) activation plays an essential role in diabetic retinopathy (DR) progression. This study was aimed to explore the role of anti-RAGE antibodies (RAGE antagonists) in inhibiting DR progression through their hypoglycemic and anti-inflammatory mechanism in diabetic retinopathy induced rats.

Methods: A total of 30 male Wistar rats were randomly divided into five group. The group was consisted of normal control group, DR group without treatment, DR group with anti-RAGE 1 ηg/kg BW, 10 ηg/kg BW, and 100 ηg/kg BW. To assess the diabetic retinopathy, fundus photographs were taken every week using a camera with 16x magnification placed in front of the rat's eyes. Blood glucose was checked by the glucose oxidase-peroxidase method. Retinal TNF-α levels and VEGF were examined using an enzyme-linked immunosorbent assay (ELISA) kit.

Results: The finding of this study showed that anti-RAGE treatment at dose of 10 and 100 ηg/kg BW, HbA1c levels were significantly higher (p< 0.05) compared to the normal control group but significantly lower (p< 0.05) than in the diabetes group. The mean blood vessel diameter in the DR+anti-RAGE 10 and 100 ηg/kg BW groups was significantly lower than in the diabetic retinopathy group (p< 0.05). The administration of anti-RAGE 10 and 100 ηg/kg BW showed the ability to significantly reduce VEGF levels compared to the DR group (p< 0.05).

Discussion: This study revealed at doses of 10 and 100 ηg/kg BW, anti-RAGE antibodies improved diabetic retinopathy in Wistar rats through hypoglycemic effects and anti-inflammatory mechanisms.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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