Justin M. Stilwell, Alvin C. Camus, Ethan T. Woodyard, Cynthia Ware, Thomas G. Rosser, Mackenzie A. Gunn, Adrián López-Porras, Lester H. Khoo, David J. Wise, Matt J. Griffin
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引用次数: 0
摘要
目的:研究斑点槽鲶(Ictalurus punctatus)和杂交槽鲶(Channel Catfish × Blue Catfish I. furcatus)的增生性鳃病(PGD)是由粘虫Henneguya ictaluri引起的。尽管有证据表明杂交鲶鱼的ictaluri传播减少，寄生虫发育受损，但PGD仍然发生在杂交生产系统中。先前临床PGD病例的宏基因组评估显示，除了伊氏蜱虫外，受影响的鳃组织中还有许多黏液动物。本研究的目的是探讨H. ictaluri和其他黏液动物在自然和实验诱导的PGD中的发育和病理作用。方法:采用RNAscope技术建立Henneguya种特异性原位杂交(ISH)方法。自然感染来自2019年提交的诊断病例。实验挑战涉及Channel鲶鱼和杂交鲶鱼，这些鲶鱼暴露在活跃的PGD爆发的池塘水中，并在挑战后1、7、10、12、14、16、18和20周取样。结果:设计了9个独特的ISH探针，针对临床PGD病例中鉴定的黏液动物类群的18S核糖体RNA基因的诊断变量区域。来自纯ictaluri H.， adiposa H.， postexilis H.和exilis H.感染的部分验证说明了物种特异性标记，不同黏液动物物种或鲶鱼宿主之间没有交叉反应。实验攻毒后，在海峡鲶鱼体内形成了成熟的ictaluri和postexilis疟原虫，但在杂交寄主体内未观察到成熟的疟原虫，表明杂交寄主的孢子发生受损或延迟。这些调查也证实了临床PGD病例中存在混合感染。结论:虽然ictaluri似乎是PGD的主要原因，但也存在其他黏液动物的孢子前阶段，这些黏液动物可能通过进一步改变正常的鳃形态来促进疾病病理并加剧呼吸损害。本研究为通道鲶鱼的ictaluri和postexilis提供了分子证实和更明确的发育时间表，并支持了先前关于杂交鲶鱼的ictaluri孢子发育受损或被阻止的研究。
Species-specific in situ hybridization confirms arrested development of Henneguya ictaluri in hybrid catfish (Channel Catfish × Blue Catfish) under experimental conditions, with notes on mixed-species infections in clinical cases of proliferative gill disease from Mississippi catfish aquaculture
期刊介绍：
The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.
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