尼古丁通过调节miR-296-3p-SIRPα轴抑制巨噬细胞的吞噬能力

IF 2.6 4区医学 Q3 CELL BIOLOGY Analytical Cellular Pathology Pub Date : 2023-01-01 DOI:10.1155/2023/6306358

Zhen Liu, Fang Wang, Xiaowu Huang, Zhi Chen, Yicheng Zhao, Yawei Wang, Xiaobo Luo, Guanren Zhao

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引用次数: 0

摘要

巨噬细胞的吞噬能力与结核感染有关。尼古丁已被证明能减弱巨噬细胞的吞噬能力;然而，其潜在机制尚不清楚。本研究表明，尼古丁增加了巨噬细胞中信号调节蛋白α (SIRPα)的mRNA和蛋白表达，并增强了SIRPα mRNA的稳定性。尼古丁降低巨噬细胞中直接靶向SIRPα mRNA 3′-非翻译区(3′-UTR)的microRNA (miR)-296-3p的表达。此外，尼古丁通过调节miR-296-3p-SIRPα轴抑制巨噬细胞的吞噬能力。此外，尼古丁通过增加巨噬细胞中c-Myc的表达来降低miR-296-3p的表达。总之，我们发现尼古丁通过调节c-Myc-miR-296-3p-SIRPα信号减弱巨噬细胞的吞噬能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nicotine Suppresses Phagocytic Ability of Macrophages by Regulating the miR-296-3p-SIRPα Axis.

Phagocytic ability of macrophage is responsible for tuberculosis infection. Nicotine has been shown to attenuate the phagocytic ability of macrophage; however, the underlying mechanism remains unclear. Here, we demonstrated that nicotine increased the message RNA (mRNA) and protein expression of signal regulatory protein alpha (SIRPα) and enhanced the stability of SIRPα mRNA in macrophage. Nicotine decreased the expression of microRNA (miR)-296-3p, which directly targeted the 3'-untranslated region (3'-UTR) of SIRPα mRNA in macrophage. Furthermore, nicotine inhibited the phagocytic ability of macrophage by regulating the miR-296-3p-SIRPα axis. Moreover, nicotine decreased miR-296-3p expression via increasing c-Myc expression in macrophage. Together, we found that nicotine attenuate the phagocytic ability of macrophage by regulating the c-Myc-miR-296-3p-SIRPα signal.

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来源期刊

Analytical Cellular Pathology ONCOLOGY-CELL BIOLOGY

CiteScore

4.90

自引率

3.10%

发文量

审稿时长

16 weeks

期刊介绍： Analytical Cellular Pathology is a peer-reviewed, Open Access journal that provides a forum for scientists, medical practitioners and pathologists working in the area of cellular pathology. The journal publishes original research articles, review articles, and clinical studies related to cytology, carcinogenesis, cell receptors, biomarkers, diagnostic pathology, immunopathology, and hematology.