白腐菌Pleurotus pulmonarius CPG6中木质素过氧化物酶的纯化、表征及其在纺织合成染料脱色中的应用。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2023-03-06 DOI:10.2323/jgam.2022.05.005
Vu Dinh Giap, Hoang Thanh Duc, Pham Thi Mai Huong, Do Thi Hanh, Do Huu Nghi, Vu Dinh Duy, Dang Thu Quynh
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引用次数: 3

摘要

从生物技术的角度来看,酶是一种强大的工具,可以从几个方面帮助维持清洁的环境。酶法生物降解合成染料是一个很有前途的研究方向,因为它可以减少纺织印染厂废水的污染。木质素过氧化物酶(EC 1.11.1.14, LiP)具有较高的氧化还原电位;因此,在各种工业领域(如造纸废水处理和纺织印染废水处理)都有很大的应用前景。本研究成功地从肺侧耳菌CPG6 (pplip)分离株中纯化了一个LiP,其比活性为6.59 U mg -1。采用DEAE、Sephadex G-75和HiTrapTM Q FF三步柱层析纯化酶,纯度为17.8倍。分子量为40 kDa的酶在酸性范围内表现出更高的pH稳定性。在pH为3.0的条件下保持6小时以上,活性保持率达75%以上。纯化后的pplip能够氧化多种底物,包括戊醇和2,4-二氯苯酚,正丙醇和愈创木酚。分析了金属离子对pplip活性的影响。该研究将为脱色各种pplip染料提供基础。纯化后的ppullip可脱色35%酸蓝25 (AB25)、50%酸红129 (AB129)、72%酸蓝62 (NY3)、85%酸蓝113 (AB113)、55%雷马唑亮蓝R (RBBR)和100%活性红120 (RR120) 12小时。大多数染料脱色,但热变性酶作为阴性对照,明显不能脱色。这些结果表明,pplip在合成染料的酶基脱色中具有潜在的应用前景。关键词:脱色;木质素过氧化物酶;侧耳属pulmonarius;纺织染料。
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Purification and characterization of lignin peroxidase from white-rot fungi Pleurotus pulmonarius CPG6 and its application in decolorization of synthetic textile dyes.

From the biotechnological point of view, enzymes are powerful tools that help sustain a clean environment in several ways. The enzymatic biodegradation of synthetic dyes is a promising goal since it reduces pollution caused by textile dyeing factory wastewater. Lignin peroxidase (EC 1.11.1.14, LiP) has high redox potential; thus, it is great for application in various industrial fields (e.g., paper- waste treatment and textile dyeing wastewater treatment). In the present study, a LiP from an isolated strain Pleurotus pulmonarius CPG6 (PpuLiP) was successfully purified with a specific activity of 6.59 U mg -1. The enzyme was purified by using three-step column chromatography procedures including DEAE, Sephadex G-75, and HiTrapTM Q FF columns with 17.8-fold purity. The enzyme with a molecular weight of 40 kDa exhibited enhanced pH stability in the acidic range. The activity retention was over 75% at a pH of 3.0 for more than 6 hours. Purified PpuLiP was able to oxidize a variety of substrates including veratryl alcohol, 2,4-DCP, n propanol, and guaiacol. The effect of metal ions on PpuLiP activity was analyzed. The study will provide a ground to decolorize dyes from various groups of PpuLiP. Purified PpuLiP could decolorize 35% Acid blue 25 (AB25), 50% Acid red 129 (AB129), 72% Acid blue 62 (NY3), 85% Acid blue 113 (AB113), 55% Remazol Brilliant blue R (RBBR), and 100% Reactive red 120 (RR120) for 12 hours. Most of the dyes were decolorized, but the heat-denatured enzyme used as negative control obviously did not decolorize the tested dyes. These results indicate that the PpuLiP has potential application in enzyme-based decolorization of synthetic dyes. Keywords: Decolorization; lignin peroxidase; Pleurotus pulmonarius; textile dyes.

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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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