Bernard Vray , Johan Hoebeke , Remi Zeeuws , A. Donny Strosberg
{"title":"兔体内有限异质性抗磷胆碱抗体的诱导","authors":"Bernard Vray , Johan Hoebeke , Remi Zeeuws , A. Donny Strosberg","doi":"10.1016/0161-5890(78)90120-7","DOIUrl":null,"url":null,"abstract":"<div><p>Repeated injections in rabbits of pneumococcal vacxine strains R 36 A induce antibodies of restricted heterogeneity as evidences by electrophoresis and isoelectric focusing. An immunoadsorbent containing purified C-polysaccharide was used to isolate and characterize the induced antibodies. Specificity for phosphorylcholine was demonstrated by hapten elution and by inhibition of precipitation. Addition of the ligand caused an enhancement of the intrinsic fluorescence of the anti-phosphorylcholine antibodies. The association constants determined by fluorometric titration were of the order of 10<sup>3</sup><em>M</em><sup>−1</sup> and were only slightly influenced by temperature.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1978-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90120-7","citationCount":"2","resultStr":"{\"title\":\"Induction of anti-phosphorylcholine antibodies of restricted heterogeneity in rabbits\",\"authors\":\"Bernard Vray , Johan Hoebeke , Remi Zeeuws , A. Donny Strosberg\",\"doi\":\"10.1016/0161-5890(78)90120-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Repeated injections in rabbits of pneumococcal vacxine strains R 36 A induce antibodies of restricted heterogeneity as evidences by electrophoresis and isoelectric focusing. An immunoadsorbent containing purified C-polysaccharide was used to isolate and characterize the induced antibodies. Specificity for phosphorylcholine was demonstrated by hapten elution and by inhibition of precipitation. Addition of the ligand caused an enhancement of the intrinsic fluorescence of the anti-phosphorylcholine antibodies. The association constants determined by fluorometric titration were of the order of 10<sup>3</sup><em>M</em><sup>−1</sup> and were only slightly influenced by temperature.</p></div>\",\"PeriodicalId\":13265,\"journal\":{\"name\":\"Immunochemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0161-5890(78)90120-7\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0161589078901207\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0161589078901207","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Induction of anti-phosphorylcholine antibodies of restricted heterogeneity in rabbits
Repeated injections in rabbits of pneumococcal vacxine strains R 36 A induce antibodies of restricted heterogeneity as evidences by electrophoresis and isoelectric focusing. An immunoadsorbent containing purified C-polysaccharide was used to isolate and characterize the induced antibodies. Specificity for phosphorylcholine was demonstrated by hapten elution and by inhibition of precipitation. Addition of the ligand caused an enhancement of the intrinsic fluorescence of the anti-phosphorylcholine antibodies. The association constants determined by fluorometric titration were of the order of 103M−1 and were only slightly influenced by temperature.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
