{"title":"[从酸性奶酪乳清中生产碱性蛋白酶]。","authors":"A E Massucco, L A Mazza, A P Balatti","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The use of acid cheese whey as an essential substrate for the production of alkaline protease was studied by growing Bacillus subtilis NRRL 3411 on different conditions of aereation. The maximum enzymatic activity of 17 000 proteolytic units was obtained in the following conditions: 200 RPM, air flow of 1 v/v minutes (KLa 37 h-1), separate sterilization of the medium components and periodical additions of peptone.</p>","PeriodicalId":76441,"journal":{"name":"Revista de la Asociacion Argentina de Microbiologia","volume":"10 1","pages":"14-19"},"PeriodicalIF":0.0000,"publicationDate":"1978-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Production of alkaline protease from acid cheese whey].\",\"authors\":\"A E Massucco, L A Mazza, A P Balatti\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The use of acid cheese whey as an essential substrate for the production of alkaline protease was studied by growing Bacillus subtilis NRRL 3411 on different conditions of aereation. The maximum enzymatic activity of 17 000 proteolytic units was obtained in the following conditions: 200 RPM, air flow of 1 v/v minutes (KLa 37 h-1), separate sterilization of the medium components and periodical additions of peptone.</p>\",\"PeriodicalId\":76441,\"journal\":{\"name\":\"Revista de la Asociacion Argentina de Microbiologia\",\"volume\":\"10 1\",\"pages\":\"14-19\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista de la Asociacion Argentina de Microbiologia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista de la Asociacion Argentina de Microbiologia","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
通过在不同曝气条件下培养枯草芽孢杆菌NRRL 3411，研究了酸性干酪乳清作为碱性蛋白酶生产的必需底物。在转速200 RPM、空气流量1 v/v min (KLa 37 h-1)、培养基组分单独灭菌和定期添加蛋白胨的条件下，酶活性最高可达17000个蛋白水解单位。
[Production of alkaline protease from acid cheese whey].
The use of acid cheese whey as an essential substrate for the production of alkaline protease was studied by growing Bacillus subtilis NRRL 3411 on different conditions of aereation. The maximum enzymatic activity of 17 000 proteolytic units was obtained in the following conditions: 200 RPM, air flow of 1 v/v minutes (KLa 37 h-1), separate sterilization of the medium components and periodical additions of peptone.
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