{"title":"[尿液中类固醇偶联物的完全酶解研究(作者译)]。","authors":"V Graef, M Fuchs","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The incubation of urine with beta-glucuronidase/arylsuphatase from Helix pomatia results in the incomplete hydrolysis of urinary steroid conjugates, because inhibitors of these enzymes are present in urine; The inhibitors can be separated from the steroid conjugates by chromatography of the urine on a column of Amberlite XAD-2. By this method a complete enzymatic hydrolysis of urinary steroid conjugates is possible in 24 hours at 37 degrees C or in 2 hours at 55 degrees C.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 4","pages":"163-7"},"PeriodicalIF":0.0000,"publicationDate":"1975-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Studies on the complete enzymatic hydrolysis of steroid conjugates in urine (author's transl)].\",\"authors\":\"V Graef, M Fuchs\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The incubation of urine with beta-glucuronidase/arylsuphatase from Helix pomatia results in the incomplete hydrolysis of urinary steroid conjugates, because inhibitors of these enzymes are present in urine; The inhibitors can be separated from the steroid conjugates by chromatography of the urine on a column of Amberlite XAD-2. By this method a complete enzymatic hydrolysis of urinary steroid conjugates is possible in 24 hours at 37 degrees C or in 2 hours at 55 degrees C.</p>\",\"PeriodicalId\":23822,\"journal\":{\"name\":\"Zeitschrift fur klinische Chemie und klinische Biochemie\",\"volume\":\"13 4\",\"pages\":\"163-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1975-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift fur klinische Chemie und klinische Biochemie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur klinische Chemie und klinische Biochemie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[Studies on the complete enzymatic hydrolysis of steroid conjugates in urine (author's transl)].
The incubation of urine with beta-glucuronidase/arylsuphatase from Helix pomatia results in the incomplete hydrolysis of urinary steroid conjugates, because inhibitors of these enzymes are present in urine; The inhibitors can be separated from the steroid conjugates by chromatography of the urine on a column of Amberlite XAD-2. By this method a complete enzymatic hydrolysis of urinary steroid conjugates is possible in 24 hours at 37 degrees C or in 2 hours at 55 degrees C.
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