Ibifiri Briggs Prekuna, M. Nchang, W. Mathew, Ikechukwu James Joseph
{"title":"尼日利亚两个地方山羊品种KiSS-1基因的测序、多态性和系统发育特征","authors":"Ibifiri Briggs Prekuna, M. Nchang, W. Mathew, Ikechukwu James Joseph","doi":"10.2298/bah2003283b","DOIUrl":null,"url":null,"abstract":"KiSS-1 gene encodes a protein product kisspeptin which are intense inducers\n of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) secretion\n in various mammalian species through its receptor GPR54 (G protein-coupled\n receptor-54). A total of 100 goat compromising of Red Sokoto (n = 72) and\n Sahel (n = 28) breeds were used to detect single nucleotide polymorphisms\n (SNPs) in the intronic region of the KiSS-1 gene by sequencing and\n investigate their relationship with other goat breeds. Nucleotide sequence\n analysis revealed five novel SNPs (g.1745G>A present in Red Sokoto,\n g.1776G>A, g.1827A>G, g.1857T>C and g.2208T>C present in Red Sokoto and\n Sahel breeds). To obtain a correct phylogenetic relationship between goat\n breeds, nucleotide sequences were compared to other sequences in NCBI\n database using a BLASTn algorithm and retrieved for further analysis.\n Neighbour-joining phylogenetic relationship tree constructed revealed two\n distinct clusters with ancestral lineage of 100% identity. Nigerian goat\n breeds (Red Sokoto and Sahel) clustered into a clade with Indian goat breeds\n (Ganjam and Osmanabadi) while the second cluster involved eight other goat\n breeds. Genetic distance estimate revealed high genetic similarity between\n Red Sokoto and Sahel breeds as observed in their genetic distance value of\n 0.003. The nucleotide sequences of the two Nigerian goat breeds (Red Sokoto\n and Sahel) for KiSS-1 gene were submitted to GenBank database and have\n accession numbers: MN122316 and MN122317, respectively. The analysis of\n polymorphism in KiSS-1 gene indicates that genetic variation exists in the\n goat breeds studied. Therefore, attempts can be made to investigate the\n association of these polymorphism with reproductive traits in Nigerian goat\n breeds.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"329 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Sequencing, polymorphism and phylogenetic characterisation of KiSS-1 gene in two Nigerian indigenous goat breeds\",\"authors\":\"Ibifiri Briggs Prekuna, M. Nchang, W. Mathew, Ikechukwu James Joseph\",\"doi\":\"10.2298/bah2003283b\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"KiSS-1 gene encodes a protein product kisspeptin which are intense inducers\\n of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) secretion\\n in various mammalian species through its receptor GPR54 (G protein-coupled\\n receptor-54). A total of 100 goat compromising of Red Sokoto (n = 72) and\\n Sahel (n = 28) breeds were used to detect single nucleotide polymorphisms\\n (SNPs) in the intronic region of the KiSS-1 gene by sequencing and\\n investigate their relationship with other goat breeds. Nucleotide sequence\\n analysis revealed five novel SNPs (g.1745G>A present in Red Sokoto,\\n g.1776G>A, g.1827A>G, g.1857T>C and g.2208T>C present in Red Sokoto and\\n Sahel breeds). To obtain a correct phylogenetic relationship between goat\\n breeds, nucleotide sequences were compared to other sequences in NCBI\\n database using a BLASTn algorithm and retrieved for further analysis.\\n Neighbour-joining phylogenetic relationship tree constructed revealed two\\n distinct clusters with ancestral lineage of 100% identity. Nigerian goat\\n breeds (Red Sokoto and Sahel) clustered into a clade with Indian goat breeds\\n (Ganjam and Osmanabadi) while the second cluster involved eight other goat\\n breeds. Genetic distance estimate revealed high genetic similarity between\\n Red Sokoto and Sahel breeds as observed in their genetic distance value of\\n 0.003. The nucleotide sequences of the two Nigerian goat breeds (Red Sokoto\\n and Sahel) for KiSS-1 gene were submitted to GenBank database and have\\n accession numbers: MN122316 and MN122317, respectively. The analysis of\\n polymorphism in KiSS-1 gene indicates that genetic variation exists in the\\n goat breeds studied. Therefore, attempts can be made to investigate the\\n association of these polymorphism with reproductive traits in Nigerian goat\\n breeds.\",\"PeriodicalId\":249404,\"journal\":{\"name\":\"Biotehnologija u stocarstvu\",\"volume\":\"329 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotehnologija u stocarstvu\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2298/bah2003283b\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotehnologija u stocarstvu","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2298/bah2003283b","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
KiSS-1基因编码的蛋白产物kisspeptin通过其受体GPR54 (G蛋白偶联受体-54)在多种哺乳动物体内强烈诱导黄体生成素(LH)和促卵泡激素(FSH)的分泌。利用红索科托山羊(72)和萨赫勒山羊(28)共100只山羊,通过测序检测了KiSS-1基因内子区域的单核苷酸多态性(snp),并研究了它们与其他山羊品种的关系。核苷酸序列分析发现5个新snp (G . 1745g >A, G . 1776g >A, G . 1827a >G, G . 1857t >C和G . 2208t >C)。为了获得山羊品种间正确的系统发育关系,利用BLASTn算法将核苷酸序列与NCBI数据库中的其他序列进行比较,并检索核苷酸序列进行进一步分析。构建的亲缘亲缘关系树显示出两个不同的群体,其祖先谱系同源性为100%。尼日利亚山羊品种(Red Sokoto和Sahel)与印度山羊品种(Ganjam和Osmanabadi)聚集在一个分支中,而第二个集群涉及其他八个山羊品种。遗传距离估计显示,红索科托和萨赫勒品种之间的遗传相似性很高,遗传距离值为0.003。将2个尼日利亚山羊品种(Red Sokoto和Sahel)的KiSS-1基因核苷酸序列提交到GenBank数据库,其登录号分别为MN122316和MN122317。KiSS-1基因多态性分析表明,所研究的山羊品种存在遗传变异。因此,可以尝试研究这些多态性与尼日利亚山羊品种繁殖性状的关系。
Sequencing, polymorphism and phylogenetic characterisation of KiSS-1 gene in two Nigerian indigenous goat breeds
KiSS-1 gene encodes a protein product kisspeptin which are intense inducers
of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) secretion
in various mammalian species through its receptor GPR54 (G protein-coupled
receptor-54). A total of 100 goat compromising of Red Sokoto (n = 72) and
Sahel (n = 28) breeds were used to detect single nucleotide polymorphisms
(SNPs) in the intronic region of the KiSS-1 gene by sequencing and
investigate their relationship with other goat breeds. Nucleotide sequence
analysis revealed five novel SNPs (g.1745G>A present in Red Sokoto,
g.1776G>A, g.1827A>G, g.1857T>C and g.2208T>C present in Red Sokoto and
Sahel breeds). To obtain a correct phylogenetic relationship between goat
breeds, nucleotide sequences were compared to other sequences in NCBI
database using a BLASTn algorithm and retrieved for further analysis.
Neighbour-joining phylogenetic relationship tree constructed revealed two
distinct clusters with ancestral lineage of 100% identity. Nigerian goat
breeds (Red Sokoto and Sahel) clustered into a clade with Indian goat breeds
(Ganjam and Osmanabadi) while the second cluster involved eight other goat
breeds. Genetic distance estimate revealed high genetic similarity between
Red Sokoto and Sahel breeds as observed in their genetic distance value of
0.003. The nucleotide sequences of the two Nigerian goat breeds (Red Sokoto
and Sahel) for KiSS-1 gene were submitted to GenBank database and have
accession numbers: MN122316 and MN122317, respectively. The analysis of
polymorphism in KiSS-1 gene indicates that genetic variation exists in the
goat breeds studied. Therefore, attempts can be made to investigate the
association of these polymorphism with reproductive traits in Nigerian goat
breeds.