螳螂虾的基因识别方法与DNA杆编码氧氧化酶1基因(CO1)

Muhammad Dailami, Dandi Saleky, A. H. A. Toha, L. P. I. Agamawan
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引用次数: 1

摘要

螳螂虾是种类非常丰富的海洋生物之一。这一类群在形态上的相似性使其在形态学上难以识别。因此,使用DNA条形码方法进行鉴定。本研究的目的是利用分子技术鉴定马诺瓦里水域的螳螂虾种类。用LCO和HCO引物扩增CO1基因片段。利用MEGA X软件分析所得DNA序列的同源性、系统发育和遗传距离。共获得Manokwari螳螂虾CO1基因片段537个碱基对。GenBank同源性分析结果显示,该序列与Gonodactylus属同源性最高,为91.18%。系统发育树分析与遗传距离分析结果一致,并与GenBank数据的同源性分析相互支持。
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Identifikasi Genetik Udang Mantis Dengan Pendekatan DNA Barcoding Gen Sitokrom Oksidase 1 (CO1)
Mantis shrimp is one of the marine organisms that has a very abundant diversity of species. The similarity of morphology of this group make it difficult to identify morphologically. Therefore, identification was carried out using a DNA barcoding approach. The purpose of this study was to identify the mantis shrimp species from Manokwari waters using molecular techniques. The CO1 gene fragments were amplified by using LCO and HCO primers. The DNA sequences obtained were analyzed for homology, phylogeny, and genetic distance using MEGA X software. A total of 537 base pairs of CO1 gene fragments of mantis shrimp from Manokwari were obtained. The results of the homology analysis on GenBank, show the highest similarity of 91.18% with sequences from the genus Gonodactylus. Analysis of phylogenetic tree and genetic distance showed the same results and supported each other with homology analysis on GenBank data.
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