Isolation and Characterization Bacillus sp. Producing Cellulase Enzymes from Hanura Mangrove

In the mangrove ecosystem, there are cellulolytic bacteria that act to decompose cellulose in nature. This study aims to obtain cellulolytic bacteria from mangrove forests in Hanura village. Bacterial isolation was carried out using Sea Water Complete Agar (SWCA) media which added Carboxymethyl Cellulose 0.5% (CMC). Characterization of isolates included colony and cell morphology, pH and NaCl stress test, pathogenicity test, and metal influence test on cellulolytic activity. The results of the study obtained 38 bacterial isolates. 16 isolates among them were cellulolytic bacteria and were able to grow well on pH (7 and 10) and NaCl (0%, 3% and 6%) stress media. IBK3, ID2K1 and IA2K3 isolates are non-pathogenic bacteria with rod-positive Gram-positive properties. IBK3 had the highest cellulolytic index of7.36 and grew well on the addition of Fe, Al, Pb and Cu metals.