A spectrophotometric method for the quantification of clotrimazole from polymeric nanoparticles to Candida albicans vaginal infections treatment

In this study, a spectrophotometric method was developed in order to quantify CLT from polymeric nanoparticles of poly(lactic-glycolic acid) (PLGA) modified on the surface with chitosan (CTS) for vaginal administration in the treatment of vaginitis. The parameters of specificity, linearity, repeatability, quantification and detection limits were evaluated. The proposed dissolution medium was Simulated Vaginal Fluid solution pH= 4.2 with Sodium Lauryl Sulfate 0.5% (p/v). The wavelength used for CLT quantification was 265 nm. The results obtained meet the acceptance criteria specified in the Analytical Method Validation Guide (García et al., 2002). In addition, the spectrophotometric method developed allowed us to determine that the percentage of CLT encapsulated in the nanoparticles was 85.64% (w/w). Finally, it is concluded that the analytical method developed is reliable, low cost and easy to perform to quantify CLT from polymeric nanoparticles of PLGA and CTS.