罗马尼亚猕猴桃育种计划——15种雄性杂交选择传粉媒介的初步研究

L. Iliescu, V. Popa, N. Meena, F. Stănică
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摘要

猕猴桃是一种可以在罗马尼亚种植的新水果。1993年,意大利和罗马尼亚共同启动了猕猴桃育种计划。对于传粉者(雄)猕猴桃,育种计划包括选择花期长、花粉发芽率高的优良植株。本研究的目的是评价15个罗马尼亚猕猴桃杂交种的花粉粒,以确定最适合猕猴桃雌性选种的传粉者。在授粉期间(2018-2020)收集新鲜花粉粒,在培养皿中室温保存12小时,然后置于15%蔗糖溶液中发芽。在不同的时间间隔:4、8、12和24小时分别进行多次测量。选择4个基因型R0P3、R0P6、R2P8和R3P9进行了进一步的田间试验,包括人工授粉和与雌性猕猴桃选材的相容性试验。
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ROMANIAN KIWIFRUIT BREEDING PROGRAM - PRELIMINARY STUDY OF FIFTEEN MALE HYBRIDS FOR SELECTION AS POLLINATORS
Kiwi is a new fruit that can be grown in Romania. A common Italian-Romanian kiwifruit breeding program was initiated in 1993. For pollinator (male) kiwi plants, breeding programs involve the selection of elite plants with long flowering period and high pollen germination rate. The aim of this study was to evaluate the pollen grains from fifteen Romanian kiwifruit hybrids for identifying the most suitable pollinators for kiwi female selections released from our breeding program. The fresh pollen grains were collected during pollination period (2018-2020), in Petri dishes, maintained at room temperature for 12 hours, and then placed in 15% sucrose solution for germination. At different time interval: 4, 8, 12 and respectively, 24 hours, several measurements were done. Four genotypes – R0P3, R0P6, R2P8 and R3P9, which recorded over 90% germinability after 24 hours, have been selected for further field tests, including artificial pollination and compatibility tests with the kiwi female selections.
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