G. Salzman, J. M. Crowell, B. J. Price, K. M. Hansen, M. Ingram, P. Mullaney
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Scattering techniques to detect and record various human cell types
Light-scattering techniques represent an appealing approach to the flow-system analysis and sorting of mammalian cells because they do not require fixation and staining, procedures that kill the cells and can introduce artifacts. A flow-system instrument has been developed1,2 at the Los Alamos Scientific Laboratory in which the intensity of light scattered by a cell as it passes through a focused laser beam is measured simultaneously at up to 32 angles between 0° and 21° with respect to the laser beam, and the scatter pattern is transferred to a computer for analysis.
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