生物标志物免疫测定的多重技术

H. Ahsan, R. Ahmad
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引用次数: 7

摘要

从单个样品中同时测量不同物质是在几种应用中实现高效和高通量检测的新兴领域。尽管免疫分析技术已经建立,并且优于其他筛选分析平台,但免疫分析的挑战之一是多路复用。虽然ELISA仍然通常用于表征单一分析物,但实验室和组织正朝着多重免疫测定的方向发展。新型生物标志物的验证及其合并到多重免疫测定中,为在单个样品中同时测量多种分析物提供了前景,从而最大限度地降低了成本、时间和样品。因此,临床科学的技术进步有助于检测样品中的分析物或生物标志物的鉴定。然而,分析生物分析仪价格昂贵,只能检测少量或类型的分析物。从单个样品中同时测量不同物质(称为多路复用)对于病理或毒理学样品的定量已变得越来越重要。虽然多重检测比传统检测有许多优点,但也存在一些可能引起临床医生和研究人员担忧的问题。因此,这些多路复用系统仍处于发展的早期阶段,面临着许多挑战。
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Multiplex Technology for Biomarker Immunoassays
The simultaneous measurement of different substances from a single sample is an emerging area for achieving efficient and high-throughput detection in several applications. Although immunoanalytical techniques are established and advantageous over alternative screening analytical platforms, one of the challenges for immunoassays is multiplexing. While ELISA is still commonly used to characterise a single analyte, laboratories and organisations are moving towards multiplex immunoassays. The validation of novel biomarkers and their amalgamation into multiplex immunoassays confers the prospects of simultaneous measurement of multiple analytes in a single sample, thereby minimising cost, time and sample. Therefore, the technological advancement in clinical sciences is helpful in the identification of analytes or biomarkers in test samples. However, the analytical bioanalysers are expensive and capable of detecting only a small amount or type of analytes. The simultaneous measurement of different substances from a single sample called multiplexing has become increasingly important for the quantification of pathological or toxicological samples. Although multiplex assays have many advantages over conventional assays, there are also problems that may cause apprehension among clinicians and researchers. Hence, many challenges still remain for these multiplexing systems which are at early stages of development.
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