用DPPH(2,2-二苯基-1-苦味酰肼)法测定96%乙醇提取物马尾藻的抗氧化活性

Dita Riwanti
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引用次数: 2

摘要

水仙是一种具有抗氧化潜力的植物。研究了马尾藻96%乙醇提取物的抗氧化活性。以马尾藻为原料,采自马都拉州苏梅内普县塔兰戈区卡布比亚村Jeruk Porot Hamlet,以96%乙醇为溶剂,采用浸渍法提取。此外,对褐藻96%乙醇提取物在156、490、501和840 mg/ml浓度下的抗氧化活性进行了测试。阳性对照采用抗坏血酸。采用DPPH(2,2-二苯基-1-苦味基Hidrazil)法,紫外-可见分光光度法,在516 nm波长下进行了96%乙醇提取物的抗氧化活性测定。结果表明,96%乙醇提取物的抗氧化活性为492,9732 mg/ml,而维生素C的IC50值为2.9498 mg/ml,表明96%乙醇提取物的抗氧化活性较抗坏血酸弱
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ANTI OXIDANT ACTIVITY OF 96% ETHANOL EXTRACT Sargassum polycystum WITH DPPH (2,2-diphenyl-1-picrylhydrazyl) METHOD USING SPECTROPHOTOMETRIC UV-VIS
S.polycystum is a plant that has potential as an antioxidant. The purpose of this study was to determine the antioxidant activity of the 96% ethanol extract of Sargassum polycystum. Sargassum polycystum was obtained from Jeruk Porot Hamlet, Cabbiya Village, Talango District, Sumenep Regency, Madura and extracted by maceration method using 96% ethanol as solvent. Furthermore, the antioxidant activity test of 96% ethanol extract of brown seaweed S. polycystum using concentrations of 156, 490, 501 and 840 mg/ml. The positive control used was ascorbic acid. Antioxidant activity test of 96% ethanol extract of S. polycystum was carried out using the DPPH (2,2-Diphenyl-1-picryl Hidrazil) method with UV-Vis spectrophotometry at a wavelength of 516 nm. The results showed that 96% ethanol extract of brown seaweed S. polycystum had antioxidant activity as indicated by an IC50 value of 492,9732 mg/ml, while vitamin C had an IC50 value of 2.9498 mg/ml so that 96% ethanol extract of Sargassum polycystum had weak antioxidant activity compared to ascorbic acid
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