Way Kambas国家公园大象训练中心圈养的雄性和雌性苏门答腊象的细胞遗传学比较研究

P. Priyambodo, E. L. Rustiati
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引用次数: 1

摘要

苏门答腊象(象maximus sumatranus)是Way Kambas国家公园(WKNP)五大哺乳动物之一。细胞遗传学研究是苏门答腊象保护工作的一部分,特别是在保护遗传领域。采集大象训练中心(ETC) WKNP圈养苏门答腊象的血液进行核型分析。采用低渗溶液预处理细胞的挤压技术对人工饲养的苏门答腊象染色体进行了分析。采用吉氏染色法对捕获的大象血细胞进行核型研究。圈养的苏门答腊象雌雄二倍体数均为56个。核型分析显示,男性和女性的总基数(FN)和常染色体基数(FNa)不同。雌性苏门答腊象的染色体FN = 8, FNa = 6,雄性苏门答腊象的染色体FN = 7, FNa = 6。X染色体的核型为大的亚稳中心,Y染色体的核型为小的远中心。本文提出的核型将与ETC WKNP所有圈养苏门答腊象的DNA指纹图谱分析相结合,为今后的育种政策提供参考。
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Comparative Cytogenetic Study on Male and Female Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park
Sumatran elephant (Elephas maximus sumatranus) was one of five big mammals in Way Kambas National Park (WKNP). Cytogenetic study was a part of conservation effort for sumatran elephant, especially in conservation genetic field. Captive sumatran elephant’s blood in Elephant Training Center (ETC) WKNP was collected to karyotype analysis. Captive sumatran elephant’s chromosome was analyzed by squashing technique with pretreating cells in a hypotonic solution. The study of karyotypes were made by giemsa staining applied to captive elephant’s blood cell. The diploid number of captive sumatran elephant was 56 both in male and female. The karyotype analysis performed different fundamental number in male and female, both of total fundamental number (FN) and autosomal fundamental number (FNa). Chromosome of female sumatran elephant showed the FN = 8, and FNa = 6, but in male sumatran elephant FN = 7, and FNa = . The karyotypes of X chromosome was large submetacentric and Y chromosome was small acrocentric.. The karyotype presented here will compilled with DNA fingerprinting analysis of all captive sumatran elephant in ETC WKNP as references in future breeding policy.
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