Karakterisasi dan Pengujian Aktivitas Antimikroba Minuman Probiotik Antanan (Centella asiatica L.)

Antanan are medicinal plants which contain alkaloids, saponins, steroids, terpenoids, carbohydrates, and reducing sugars. But, on the other hand, the use of them are rarely utilized. The purposes of this research were to determine the characteri stics of antanan probiotic drink and examine the effectiveness of antanan probiotic as an antimicrobial for the Staphylococcus aureus and Escherichia coli . Steps of this research were the making of antanan probiotic drink with skim milk concentration variation treatment, characterization of the raw materials and antanan probiotic, and antimicrobial test of antanan probiotic . The results showed that antanan probiotic with a concentration of skim milk 5%, 7%, and 9% had a fat content of between 0.024% - 0.178%, protein content of 3.708% - 8,589%, ash content of 0.544% - 0.738%, and the total acid of 0.951% - 1.198%. These results were met with National Standard of Indonesia (SNI 2981:2009) about Yoghurt. The test results of antimicrobial activity showed that antanan probiotic can inhibit the growth of bacteria Staphylococcus aureus and Escherichia coli . ABSTRAK Antanan merupakan tanaman obat yang mengandung alkaloid, saponin, steroid, terpenoid, karbohidrat, dan gula pereduksi namun demikian pemanfaatannya masih belum banyak. Tujuan penelitian ini adalah mengetahui karakteristik minuman probiotik antanan dan mengetahui efektivitas minuman probiotik antanan sebagai antimikroba bagi bakteri Staphylococcus aureus dan Escherichia coli . Tahapan penelitian dilakukan dengan membuat minuman probiotik antanan dengan variasi konsentrasi susu skim. Selanjutnya dilakukan karakterisasi terhadap bahan baku, jus antanan dan minuman probiotik antanan serta uji antimikroba dari minuman probiotik antanan. Hasil pengujian menunjukkan bahwa minuman probiotik antanan dengan konsentrasi susu skim 0%, 5%, 7%, dan 9% memiliki kandungan lemak antara 0,024%-0,178%, kadar protein3,708%- 8,589%, kadar abu 0,268% -0,738%, dan total asam 0,403%-1,1981%.Hasil tersebut telah memenuhi standar SNI 2981:2009 tentang yoghurt. Hasil uji aktivitas antimikroba menunjukkan bahwa minuman probiotik antanan dapat menghambat pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli . Minuman probiotik antanan dengan konsentrasi susu skim 5% merupakan konsentrasi terbaik untuk menghambat pertumbuhan mikroba. Kata kunci :Antanan, antimikroba, Escherichia coli , Staphylococcus aureus , minuman probiotik