山羊感染布鲁氏菌的实验研究。细菌学、血清学和组织病理学研究]。

C García-Carbrillo, R Casas-Olascoaga, A Cuba-Caparó, N Lucero, B Szyfres
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引用次数: 0

摘要

研究了成年公山羊对布鲁氏菌感染的敏感性。15只年龄在22至34个月之间的山羊和15只公绵羊,用最近从一只公绵羊附睾炎病例中分离出来的10(9)个卵泡杆菌株进行结膜接种。接种后78 d杀5只山羊和5只公绵羊,之后每隔2个月杀1组。接种后33天和61天,从一只公山羊的精液中分别检出了该菌。接种后78天处死的5只山羊器官中均含有布鲁氏菌。另外两组的精液和组织在整个观察期间保持阴性。从14只公羊的精液中获得7个培养物作为接种对照。临床上仅在一只公山羊中观察到附睾炎,尽管有六只公山羊出现了肉眼可见的病变。15只接种公羊中有7只出现附睾炎的临床症状。在山羊体内,补体固定和免疫扩散检测到的抗体在接种后80天消失,而公羊在189天的观察期内出现了反应。结果表明,公山羊的感染是短暂的,在该病流行病学中的作用可以忽略不计。
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[Experimental infection of goats with Brucella ovis. Bacteriological serological and histopathological studies].

The susceptibility of adult male goats to Brucella ovis infection was studied. Fifteen goats and fifteen rams both of ages ranging from 22 to 34 months were inoculated conjunctivally with 10(9) cells of B. ovis strain recently isolated from a case of ram epididymitis. Five goats and five rams were killed 78 days after inoculation and similar groups were killed at two month intervals thereafter. B. ovis was recovered from semen of a male goat, 33 and 61 days after inoculation. The five goats sacrificed 78 days after inoculation contained Brucella in their organs. The semen and the tissues of the two other groups remained culturally negative throughout the observation period. Seven cultures were obtained from the semen of 14 rams used comparatively as inoculated controls. Epididymitis was clinically observed only in one male goat although under six presented macroscopic lesions. Seven rams out of the 15 inoculated showed clinical symptoms of epididymitis. Antibodies detectable by complement fixation and immunodiffusion disappeared 80 days after inoculation in goats, while rams reacted during the 189 days period of observation. It is concluded that the B. ovis infection in male goats is transient and the role that they may play in the epizootiology of the disease is negligible.

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