{"title":"tepc15小鼠骨髓瘤蛋白和豚鼠抗卵磷脂血清免疫沉淀磷胆碱脂质的研究","authors":"Barbara Niedieck, Ursula Kuck, Hedda Gardemin","doi":"10.1016/0161-5890(78)90076-7","DOIUrl":null,"url":null,"abstract":"<div><p>The BALB/c mouse myeloma protein TEPC 15 (T 15) and sera of guinea pigs immunized with lecithin (lec) precipitate in agarose gels with mixed micelles bearing the phosphorylcholine lipids (PC-lipids) lec, lysolecithin and sphingomyelin. The T 15 IgA and the guinea pig anti-lee antibody are both totally inhibited in agarose gels containing phosphorylcholine. The advantages of defined PC-lipids in contrast to the commonly employed complex antigens for the detection of anti-PC immunoglobulins are discussed.</p></div>","PeriodicalId":13265,"journal":{"name":"Immunochemistry","volume":"15 7","pages":"Pages 471-475"},"PeriodicalIF":0.0000,"publicationDate":"1978-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0161-5890(78)90076-7","citationCount":"10","resultStr":"{\"title\":\"On the immune precipitation of phosphorylcholine lipids with tepc 15 mouse myeloma protein and with anti-lecithin sera from guinea pigs\",\"authors\":\"Barbara Niedieck, Ursula Kuck, Hedda Gardemin\",\"doi\":\"10.1016/0161-5890(78)90076-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The BALB/c mouse myeloma protein TEPC 15 (T 15) and sera of guinea pigs immunized with lecithin (lec) precipitate in agarose gels with mixed micelles bearing the phosphorylcholine lipids (PC-lipids) lec, lysolecithin and sphingomyelin. The T 15 IgA and the guinea pig anti-lee antibody are both totally inhibited in agarose gels containing phosphorylcholine. The advantages of defined PC-lipids in contrast to the commonly employed complex antigens for the detection of anti-PC immunoglobulins are discussed.</p></div>\",\"PeriodicalId\":13265,\"journal\":{\"name\":\"Immunochemistry\",\"volume\":\"15 7\",\"pages\":\"Pages 471-475\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1978-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0161-5890(78)90076-7\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0161589078900767\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0161589078900767","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
On the immune precipitation of phosphorylcholine lipids with tepc 15 mouse myeloma protein and with anti-lecithin sera from guinea pigs
The BALB/c mouse myeloma protein TEPC 15 (T 15) and sera of guinea pigs immunized with lecithin (lec) precipitate in agarose gels with mixed micelles bearing the phosphorylcholine lipids (PC-lipids) lec, lysolecithin and sphingomyelin. The T 15 IgA and the guinea pig anti-lee antibody are both totally inhibited in agarose gels containing phosphorylcholine. The advantages of defined PC-lipids in contrast to the commonly employed complex antigens for the detection of anti-PC immunoglobulins are discussed.
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