小儿急性肠胃炎病因测定的实验技术。

B D Schoub, Y R Jacobs, R M Robins-Browne, H J Koornhof, G Lecatsas, O W Prozesky
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摘要

描述了在研究实验室中用于婴儿肠胃炎微生物诊断的技术。这些技术本质上是细菌性腹泻三种发病机制的实验模型。从临床细菌学的角度来看,虽然鉴定沙门氏菌和志贺氏菌等肠病细菌没有什么困难,但用目前的常规细菌学程序区分大肠杆菌和共生菌的肠病菌株是不可能的。讨论了这些实验技术适用于常规实验室的可能性。呼肠孤病毒样颗粒是婴儿胃肠炎最重要的病毒病原体,目前,阴性染色电镜是检测呼肠孤病毒样颗粒的唯一方法。由于其相对简单,强烈建议在所有婴儿肠胃炎病例中进行该检查。
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Experimental techniques in the determination of aetiology of acute infantile gastroenteritis.

Techniques used in the research laboratory for the microbiological diagnosis of infantile gastroenteritis are described. These techniques are in essence experimental models of the three pathogenetic mechanisms of bacterial diarrhoea. From the clinical bacteriological standpoint, although there is little difficulty with the identification of enteropathic bacteria such as Salmonella and Shigella, the differentiation of enteropathic strains of Escherichia coli from commensal strains is not possible with present routine bacteriological procedures. Possible adaptations of these experimental techniques to the routine laboratory are discussed. At present, negative-staining electron microscopy is the only way in which reoviruslike particles, the most important viral pathogen in infantile gastroenteritis, may be detected. Because of its relative simplicity this examination is strongly recommended in all cases of infantile gastroenteritis.

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