{"title":"双接种番茄植株烟草花叶病毒轻、重度株的鉴定","authors":"A. C. CASSELLS, C. C. HERRICK","doi":"10.1111/j.1744-7348.1977.tb01813.x","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Using specific antisera, it was possible to identify mild (MII-16) and the 0 and 1 strains of tobacco mosaic virus (TMV) in tomato plants infected with the mild strain following challenge inoculation with the wild strains.</p>\n <p>In addition, with single lesion isolates of the MII-16 and o strains it has been shown that both strains can be assayed simultaneously in mixed isolates on <i>Nicotiana glutinosa</i> based on differences in local lesion size. The accuracy of this differential local lesion assay was c. 90% as determined by back inoculation of single lesion isolates into susceptible tomato cultivars.</p>\n </div>","PeriodicalId":7977,"journal":{"name":"Annals of Applied Biology","volume":"86 1","pages":"37-46"},"PeriodicalIF":2.2000,"publicationDate":"1977-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1744-7348.1977.tb01813.x","citationCount":"9","resultStr":"{\"title\":\"The identification of mild and severe strains of tobacco mosaic virus in doubly inoculated tomato plants\",\"authors\":\"A. C. CASSELLS, C. C. HERRICK\",\"doi\":\"10.1111/j.1744-7348.1977.tb01813.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Using specific antisera, it was possible to identify mild (MII-16) and the 0 and 1 strains of tobacco mosaic virus (TMV) in tomato plants infected with the mild strain following challenge inoculation with the wild strains.</p>\\n <p>In addition, with single lesion isolates of the MII-16 and o strains it has been shown that both strains can be assayed simultaneously in mixed isolates on <i>Nicotiana glutinosa</i> based on differences in local lesion size. The accuracy of this differential local lesion assay was c. 90% as determined by back inoculation of single lesion isolates into susceptible tomato cultivars.</p>\\n </div>\",\"PeriodicalId\":7977,\"journal\":{\"name\":\"Annals of Applied Biology\",\"volume\":\"86 1\",\"pages\":\"37-46\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"1977-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1744-7348.1977.tb01813.x\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of Applied Biology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/j.1744-7348.1977.tb01813.x\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Applied Biology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/j.1744-7348.1977.tb01813.x","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
The identification of mild and severe strains of tobacco mosaic virus in doubly inoculated tomato plants
Using specific antisera, it was possible to identify mild (MII-16) and the 0 and 1 strains of tobacco mosaic virus (TMV) in tomato plants infected with the mild strain following challenge inoculation with the wild strains.
In addition, with single lesion isolates of the MII-16 and o strains it has been shown that both strains can be assayed simultaneously in mixed isolates on Nicotiana glutinosa based on differences in local lesion size. The accuracy of this differential local lesion assay was c. 90% as determined by back inoculation of single lesion isolates into susceptible tomato cultivars.
期刊介绍：
Annals of Applied Biology is an international journal sponsored by the Association of Applied Biologists. The journal publishes original research papers on all aspects of applied research on crop production, crop protection and the cropping ecosystem. The journal is published both online and in six printed issues per year.
Annals papers must contribute substantially to the advancement of knowledge and may, among others, encompass the scientific disciplines of:
Agronomy
Agrometeorology
Agrienvironmental sciences
Applied genomics
Applied metabolomics
Applied proteomics
Biodiversity
Biological control
Climate change
Crop ecology
Entomology
Genetic manipulation
Molecular biology
Mycology
Nematology
Pests
Plant pathology
Plant breeding & genetics
Plant physiology
Post harvest biology
Soil science
Statistics
Virology
Weed biology
Annals also welcomes reviews of interest in these subject areas. Reviews should be critical surveys of the field and offer new insights. All papers are subject to peer review. Papers must usually contribute substantially to the advancement of knowledge in applied biology but short papers discussing techniques or substantiated results, and reviews of current knowledge of interest to applied biologists will be considered for publication. Papers or reviews must not be offered to any other journal for prior or simultaneous publication and normally average seven printed pages.
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