Detection of glycoprotein using fiber optic surface plasmon resonance sensors with boronic acid

In this paper, we present a tilted fiber Bragg gratings (TFBG) based surface Plasmon resonance (SPR) labelfree sensors with boronic acid derivative (ABA-PBA) as receptor molecule to detect glycoprotein with high sensitivity and selectivity. Tilted fiber Bragg gratings (TFBG) as a near infrared wavelengths detecting element can be able to excite a number of cladding modes whose properties can be detected accurately by measuring the variation of transmitted spectra. A 10° TFBG coated by 50nm gold film was manufactured to stimulate surface plasmon resonance on the surface of the sensor. The sensor was loaded with boronic acid derivative as the recognition molecule which has been widely used in various areas for the recognition matrix of diol-containing biomolecules. The proposed TFBG-SPR sensors exhibit good selectivity and repeatability with the protein concentration sensitivity up to 2.867dB/(mg/ml) and the limit of detection was 2∗10−5g/ml.