A. L. Gronlund, J. Dickinson, P. Kille, J. Harwood, R. Herbert, D. Francis, H. Rogers
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Plant WEE1 Kinase Interacts with a 14-3-3 Protein, GF14ω but a Mutation of WEE1 at S485 Alters Their Spatial Interaction
In animals, 14-3-3 proteins bind two cell cycle proteins WEE1 and CDC25 stabilising their phosphorylated
state. We report here for the first time interactions between WEE1 and 14-3-3 proteins both in vitro and in vivo in plants.
The Arabidopsis 14-3-3 family partitions into either an Epsilon or Non-Epsilon group. In a yeast 2-hybrid screen
Arabidopsis WEE1 interacted with the Non-Epsilon group. Subsequently, we focussed on Non-Epsilon GF14