声学显微镜下细胞-底物附着的观察

J. Hildebrand
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引用次数: 20

摘要

摘要:采用工作频率为1.7 GHz的扫描反射声显微镜,观察活细胞与基质之间的接触。当声学显微镜镜头聚焦在基板表面时,细胞-基板附着的位置在声学图像中显示为黑暗区域。定量分析表明,相对于细胞内的其他区域,附着位点的声阻抗增加。运动细胞的声学图像显示了附着部位阻抗的时空变化。
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Observation of Cell-Substrate Attachment with the Acoustic Microscope
Abstmcf-The scanning reflection acoustic microscope operating at 1.7 GHz is used to observe the contacts between living cells and their substrates. When the acoustic microscope lens is focused on the surface of the substrate, sites of cell-substrate attachment appear as darkened areas in the acoustic image. Quantitative analysis indicates that attachment sites have increased acoustic impedance relative to other regions within the cell. Acoustic images of moving cells show spatial and temporal changes in the impedance at the attachment site.
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