{"title":"HIIT训练课程对老年雌性大鼠心脏组织中PGC-1α、SIRT1和SIRT3基因表达的影响","authors":"Khadijeh Hassanpour, B. Abedi, Lida Moradi","doi":"10.32598/jvc.2.4.100.1","DOIUrl":null,"url":null,"abstract":"Background and Aim: Exercise improves the function of mitochondrial proteins and enzymes in old age. This study aimed to investigate the effect of a high-intensity interval training (HIIT) training course on the expression of PGC-1α, SIRT1, and SIRT3 genes in the cardiac tissue of elderly female rats. Materials and Methods: In this experimental study, 14 female Sprague-Dawley rats with a mean age of 16-18 months and a weight range of 280-320 g were randomly divided into 2 groups of 7, including the control group and the HIIT training group. The experimental group performed HIIT training for 8 weeks and 5 days a week with an intensity of 85%- 110% VO2max. Forty-eight hours after the last training session, rats were anesthetized by intraperitoneal injection of ketamine and xylazine, and the target tissue was immediately removed for examination. Shapiro-Wilk statistical test and one-way analysis of variance in SPSS software v. 22 analyzed the findings (P≥0.05). Results: The results showed that the expression of PGC-1α, SIRT1, and SIRT3 genes increased in the cardiac tissue of the experimental group compared to the control group after 8 weeks of HIIT training, but this increase was not significant (P≤0.05). Conclusion: It seems that in elderly conditions, exercise from pathways other than PGC- 1α improves mitochondrial function. However, further research is needed on the effect of HIIT exercise on the expression of these genes in cardiac tissue.","PeriodicalId":318985,"journal":{"name":"Journal of Vessels and Circulation","volume":"20 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Effect of a HIIT Training Course on the Expression of PGC-1α, SIRT1, and SIRT3 Genes in the Cardiac Tissue of Elderly Female Rats\",\"authors\":\"Khadijeh Hassanpour, B. Abedi, Lida Moradi\",\"doi\":\"10.32598/jvc.2.4.100.1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background and Aim: Exercise improves the function of mitochondrial proteins and enzymes in old age. This study aimed to investigate the effect of a high-intensity interval training (HIIT) training course on the expression of PGC-1α, SIRT1, and SIRT3 genes in the cardiac tissue of elderly female rats. Materials and Methods: In this experimental study, 14 female Sprague-Dawley rats with a mean age of 16-18 months and a weight range of 280-320 g were randomly divided into 2 groups of 7, including the control group and the HIIT training group. The experimental group performed HIIT training for 8 weeks and 5 days a week with an intensity of 85%- 110% VO2max. Forty-eight hours after the last training session, rats were anesthetized by intraperitoneal injection of ketamine and xylazine, and the target tissue was immediately removed for examination. Shapiro-Wilk statistical test and one-way analysis of variance in SPSS software v. 22 analyzed the findings (P≥0.05). Results: The results showed that the expression of PGC-1α, SIRT1, and SIRT3 genes increased in the cardiac tissue of the experimental group compared to the control group after 8 weeks of HIIT training, but this increase was not significant (P≤0.05). Conclusion: It seems that in elderly conditions, exercise from pathways other than PGC- 1α improves mitochondrial function. However, further research is needed on the effect of HIIT exercise on the expression of these genes in cardiac tissue.\",\"PeriodicalId\":318985,\"journal\":{\"name\":\"Journal of Vessels and Circulation\",\"volume\":\"20 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Vessels and Circulation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32598/jvc.2.4.100.1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Vessels and Circulation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32598/jvc.2.4.100.1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The Effect of a HIIT Training Course on the Expression of PGC-1α, SIRT1, and SIRT3 Genes in the Cardiac Tissue of Elderly Female Rats
Background and Aim: Exercise improves the function of mitochondrial proteins and enzymes in old age. This study aimed to investigate the effect of a high-intensity interval training (HIIT) training course on the expression of PGC-1α, SIRT1, and SIRT3 genes in the cardiac tissue of elderly female rats. Materials and Methods: In this experimental study, 14 female Sprague-Dawley rats with a mean age of 16-18 months and a weight range of 280-320 g were randomly divided into 2 groups of 7, including the control group and the HIIT training group. The experimental group performed HIIT training for 8 weeks and 5 days a week with an intensity of 85%- 110% VO2max. Forty-eight hours after the last training session, rats were anesthetized by intraperitoneal injection of ketamine and xylazine, and the target tissue was immediately removed for examination. Shapiro-Wilk statistical test and one-way analysis of variance in SPSS software v. 22 analyzed the findings (P≥0.05). Results: The results showed that the expression of PGC-1α, SIRT1, and SIRT3 genes increased in the cardiac tissue of the experimental group compared to the control group after 8 weeks of HIIT training, but this increase was not significant (P≤0.05). Conclusion: It seems that in elderly conditions, exercise from pathways other than PGC- 1α improves mitochondrial function. However, further research is needed on the effect of HIIT exercise on the expression of these genes in cardiac tissue.