T. Garkavenko, O. Gorbatiuk, T. G. Kozitska, V. Andriyashchuk, S. Dibkova
{"title":"用VanA和VanB酶测定饮用水中肠球菌的万古霉素耐药菌株","authors":"T. Garkavenko, O. Gorbatiuk, T. G. Kozitska, V. Andriyashchuk, S. Dibkova","doi":"10.31073/vet_biotech36-02","DOIUrl":null,"url":null,"abstract":"isolates of Enterococcus spp., to identify among them VRE-strains and to confirm the production of VanA- and VanB-enzymes. Materials and methods . 14 field isolates of Enterococcus spp. isolated from drinking water samples in the Odessa region were investigated. Sensitivity determination of experimental clinical isolates of Enterococcus spp. was performed by a disc-diffusion method using antibiotic disks according to EUCAST recommendations. Results of research and discussion . Analysis of the results of studies to determine the sensitivity of Enterococcus spp. to AMP showed that 10 strains, from the studied 14, had sensitivity to all used antibiotics, that was 71,5% of all enterococcal isolates. Polyantibiotic resistance was established in 1 strain because it was insensitive to imipenem, levofloxacin, teicoplanin, and vancomycin. Among 4 strains belonging to the VRE were identified. Among them, 2 strains confirmed the production of VanA-enzymes and in 2 strains the production of VanB-enzymes. further research to study the level of resistance to AMP at film-forming Enterococcus","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"11 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Determination of vancomicin- resistant strains (VRE) with VanA and VanB\\n enzymes among field isolates of Enterococcus spp., obtained from drinking\\n water samples\",\"authors\":\"T. Garkavenko, O. Gorbatiuk, T. G. Kozitska, V. Andriyashchuk, S. Dibkova\",\"doi\":\"10.31073/vet_biotech36-02\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"isolates of Enterococcus spp., to identify among them VRE-strains and to confirm the production of VanA- and VanB-enzymes. Materials and methods . 14 field isolates of Enterococcus spp. isolated from drinking water samples in the Odessa region were investigated. Sensitivity determination of experimental clinical isolates of Enterococcus spp. was performed by a disc-diffusion method using antibiotic disks according to EUCAST recommendations. Results of research and discussion . Analysis of the results of studies to determine the sensitivity of Enterococcus spp. to AMP showed that 10 strains, from the studied 14, had sensitivity to all used antibiotics, that was 71,5% of all enterococcal isolates. Polyantibiotic resistance was established in 1 strain because it was insensitive to imipenem, levofloxacin, teicoplanin, and vancomycin. Among 4 strains belonging to the VRE were identified. Among them, 2 strains confirmed the production of VanA-enzymes and in 2 strains the production of VanB-enzymes. further research to study the level of resistance to AMP at film-forming Enterococcus\",\"PeriodicalId\":274594,\"journal\":{\"name\":\"Bulletin \\\"Veterinary biotechnology\\\"\",\"volume\":\"11 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin \\\"Veterinary biotechnology\\\"\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.31073/vet_biotech36-02\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin \"Veterinary biotechnology\"","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31073/vet_biotech36-02","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Determination of vancomicin- resistant strains (VRE) with VanA and VanB
enzymes among field isolates of Enterococcus spp., obtained from drinking
water samples
isolates of Enterococcus spp., to identify among them VRE-strains and to confirm the production of VanA- and VanB-enzymes. Materials and methods . 14 field isolates of Enterococcus spp. isolated from drinking water samples in the Odessa region were investigated. Sensitivity determination of experimental clinical isolates of Enterococcus spp. was performed by a disc-diffusion method using antibiotic disks according to EUCAST recommendations. Results of research and discussion . Analysis of the results of studies to determine the sensitivity of Enterococcus spp. to AMP showed that 10 strains, from the studied 14, had sensitivity to all used antibiotics, that was 71,5% of all enterococcal isolates. Polyantibiotic resistance was established in 1 strain because it was insensitive to imipenem, levofloxacin, teicoplanin, and vancomycin. Among 4 strains belonging to the VRE were identified. Among them, 2 strains confirmed the production of VanA-enzymes and in 2 strains the production of VanB-enzymes. further research to study the level of resistance to AMP at film-forming Enterococcus