越南小范围管理赤潮病菌的尝试及芸香科品种/无性系耐赤潮病菌的初步筛选

V. H. Nguyen, T. Tran, T. Nguyen, T. Bui, M. Nguyen
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摘要

在越南湄公河三角洲,柑桔黄龙病(Citrus Huanglongbing, HLB)于1994年被正式宣布,1995年Bove和Garnier将其致病菌描述为Candidatus Liberibacter asiaticus。多年来,在小规模果园进行了大量的防治工作,并对防治成果进行了探讨,探讨了在小规模果园有效防治柑桔病的模式,以期延长柑桔树的生命周期,获得更好、更长的收获。此外,还收集了130份芸香科相关材料进行了HLB耐受性筛选;结果表明,HLB侵染在杏/Tac/Quat (Citrus microcarpa)和Long Co Co pummelo (Citrus maxima)上的严重程度低于柑橘和柑桔。芦科野生种/无性系Quyt Dang、Quyt rung、Cam rung、Buoi rung、Buoi Dang、Buoi Bung、Mac Run、Mac Mat、Can Thang、Quach、Nguyet Que、Kim quit、Truc、Com Ruou、Dau ba la、Ca ri、Da tu bien和Gioi Lom在传播条件下无症状,PCR检测结果为阴性。在分子研究中,设计了38条引物,用于筛选芦花科49个品种/无性系的HLB耐受性。初步结果表明,这些耐受性品种被归为D组,与筛选条件下的耐受性组有一定的匹配。
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Results on attempts in management of HLB under small scale in Vietnam and initiation in screening for HLB tolerant from varieties/clones belonging to Rutaceae
In the Mekong Delta, Vietnam, Citrus Huanglongbing (HLB) was officially announced in 1994 and its causal organism was described by Bove and Garnier in 1995 to be Candidatus Liberibacter asiaticus. Throughout the years, intensive works have been carried out for HLB control under small scale orchards and the achievements are discussed, the model for effective control of HLB under small scale which could elongate the life cycle of citrus tree for better and longer harvesting. In addition, there were 130 rutaceae related accessions had been collected and screened for HLB tolerance; the results revealed that the serverity of HLB infection was less on Hanh/Tac/Quat (Citrus microcarpa) and Long Co co pummelo (Citrus maxima) than that on orange and mandarin. The wild Rutaceae species/clones such as Quyt Dang, Quyt rung, Cam rung, Buoi Rung, Buoi Dang, Buoi Bung, Mac Run, Mac Mat, Can Thang, Quach, Nguyet Que, Kim quit, Truc, Com Ruou, Dau dau ba la, Ca ri, Da tu bien and Gioi Lom were symptomless under transmission conditions and had a negative reaction by PCR tests. In a molecular study, 38 primers have been designed and used for screening of HLB tolerance capacity of 49 varieties/clones belonging to Rutaceae. The preliminary results shown that the tolerant varieties was grouped into Group D, which somehow matched with the tolerant group screened under screenhouse conditions.
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