The establishment of detecting carbohydrate antigen 125 (CA125) with immunofluorescence chromatography

This study was designed to develop a immunofluorescence chromatography kit for detection of CA125 in human serum, and to evaluate the method performance. The fluorescent polystyrene particle conjugated with the monoclonal antibody MIC05 was used as a fluorescent label. The monoclonal antibody MIC06 and mouse anti-rabbit IgG were immobilized on the nitrocellulose membrane as the test line and control line. The linear response rang of the method to detect the serum CA125 was 0.56–1000U/ml. And the intra-and inter-assay coefficient were 5.39% and 10.3%. Total 1200 samples were tested by the immunofluorescence chromatography kit, and the overall coincidence rate was 94.63%. The positive coincidence rate was 92.48%, the negative coincidence rate was 95.64%, and the Kappa value surpassed 0.8, which indicated that there was no significant difference between the results of the immunofluorescence chromatography kit and Elisa kit. In conclusion, the immunofluorescence chromatography kit for assaying CA125 provided high sensitivity, rapid, simple operation, low cost, single-servings and can be extensively used for clinical operarions.