Roxb的转基因种子。使用硫酸(H2SO4)

Tati Suhartati, Surodjo Taat Andayani, Sugeng Wahyudiono
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摘要

甘美兰(Gmelina arborea Roxb.)种子是一种具有物理休眠的种子。这是因为在发芽过程中,坚硬的种皮阻挡了水分和气体。因此,为了打破休眠状态,需要以刻蚀的形式进行初步处理,包括使用硫酸(H2SO4)溶液。本研究旨在确定在浓度为96%的H2SO4溶液中浸泡处理能产生最佳的发芽能力。试验采用完全随机设计方法(CRD),设置5个浸泡时间处理,即对照(0分钟)、5分钟、10分钟、15分钟、20分钟,每个处理重复3次,每次重复50粒种子。结果表明,96%浓度的H2SO4浸泡对种子的萌发起始时间、最后萌发时间、80%极限萌发时间、发芽率和活力指数均有显著影响。浸泡时间为10分钟是最好的处理方法。关键词:生理休眠;预处理;划痕;浸泡时间
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Pematahan Dormansi Benih Gmelia arborea Roxb. Menggunakan Asam Sulfat (H2SO4)
Gmelina (Gmelina arborea Roxb.) seed is a type of seed that have physical dormancy. This is because water and gases prevent by the hard seed coat during the germination process. Therefore, to break dormancy, preliminary treatment in the form of scarification is required, including using sulfuric acid (H2SO4) solution. This study aims to determine the treatment of soaking in a 96% concentration of H2SO4 solution that produces the best germination ability. The study used a completely randomized design method (CRD) with 5 soaked time treatments, namely control (0 minutes), 5 minutes, 10 minutes, 15 minutes, 20 minutes and each treatment was repeated 3 times with 50 seeds for each replication. The results showed that soaked the seeds in the 96% concentration of H2SO4 solution had a significant effect on on initial time of germination, last time of germination, 80% limit time for germination, percentage of germination and vigor index. Soaking time for 10 minutes is the best treatment for scarification of gmelina seeds.   Keywords: physical dormancy; pre-treatment; scarification; soaking time
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