补充信息

Heterostructured Materials Pub Date : 2021-09-14 DOI:10.1201/9781003153078-37

Xiaolei Wu, Yuntian Zhu

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引用次数: 0

摘要

离体愈伤组织的产生。如前所述，诱导体外愈伤组织产生(Iwase等，2011)。突变型和野生型幼苗在发芽后4天切除黄化的下胚轴。突变体和野生型外植体在MS培养基上分别培养30 d，培养基中添加甘堡维生素、2%葡萄糖、0.05% 2-(N-morpholino)乙磺酸(MES)、pH 5.8 (KOH)、0.8%植物琼脂以及指定浓度的NAA和动蛋白。

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Supplementary Information Text

In Vitro Callus Production. Induction of in vitro callus production was performed as previously described (Iwase et al., 2011). Etiolated hypocotyls were excised from mutant and wild type seedlings 4 days after germination. Mutant and wild type explants were cultured for 30 days side-by-side on MS medium supplemented with Gamborg’s vitamins, 2% glucose, 0.05% 2-(N-morpholino)ethanesulfonic acid (MES), pH 5.8 with KOH, 0.8% phytoagar, and the indicated concentrations of NAA and kinetin.

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Heterostructured Materials

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