赤月牙乙酸乙酯部分的驱虫潜能屈血螨离体抗病死率的研究

Dyah Ajeng Suhita
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摘要

本研究旨在研究月牙果乙酸乙酯部位对弯血螨的体外杀虫效果。本研究采用的方法为仅测试后对照组设计。共5个处理,每个处理4个重复。在所有重复的每个处理中,使用的样本为20个H. contortus。分别于5、10、15、20、25、30、35、40、45、50、55、60分钟及培养皿中虫体全部死亡时观察和记录弯纹弓形虫的死亡率。数据分析采用方差分析,其次是邓肯检验和概率分析。结果表明,白耳果乙酸乙酯部分具有驱虫活性。本研究的结论是,最佳浓度为0.5%乙酸乙酯部分浓度,连续各观察时间LC50为1.39%;0.97%;0.70%;0.48%;0.39%;0.34%;0.31%;0.28%;0.27%;0.26%;0.25%;和0.24%,而LT50浓度为0.125%;0.25%;0.5%分别是1小时17秒、41分42秒和20分58秒。
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Anthelmintic Potential of Ethyl Acetate Fraction Berenuk (Crescentia cujete L.) Fruit Against Mortality Haemonchus contortus In Vitro
This study aims to determine the anthelmintic potency of the ethyl acetate fraction of berenuk fruit (Crescentia cujete L.) on the mortality of the Haemonchus contortus worms in vitro. The method that was used in the research was a post-test only control group design. There were five treatments and each treatment was carried out in four repetitions. The sample used was twenty H. contortus in each treatment for all replications. Observation and recording of H. contortus mortality was carried out at 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 minutes, and when all worms in the petri dish died. Data analysis using ANOVA, followed by Duncan’s Test and Probit analysis. The results showed that the ethyl acetate fraction of berenuk fruit had anthelmintic activity. The conclusion in this study is that the optimal concentration is found in the 0.5% ethyl acetate fraction concentration, the LC50 at each observation time successively is 1.39%; 0.97%; 0.70%; 0.48%; 0.39%; 0.34%; 0.31%; 0.28%; 0.27%; 0.26%; 0.25%; and 0.24%, while LT50 at a concentration of 0.125%; 0.25%; and 0.5% respectively are 1 hour 17 seconds, 41 minutes 42 seconds, and 20 minutes 58 seconds.
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