培养液中加入脑脊液对牛腺垂体体外分泌黄体生成素的影响。

P B Gradwell, R B Symington
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引用次数: 0

摘要

采用牛组织进行了六项体外试验,研究在下丘脑存在和不存在的情况下,在培养液中加入脑脊液(csf)对腺垂体组织分泌黄体生成素(LH)的影响。此外,还研究了在单独培养基和培养基+ csf中加入松果体对LH分泌的影响。当csf浓度在5%到40%之间时,当组织单独在csf中孵育时,以及当松果体加入培养系统时,对LH分泌没有明显或一致的影响。这些结果不支持csf中含有大量能促进垂体前叶LH分泌的物质的观点。
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The effect of inclusion of cerebrospinal fluid in the incubation medium on the in vitro secretion of luteinizing hormone from the bovine adenohypophysis.

Six in vitro trials were conducted using bovine tissues to study the effects of addition of cerebrospinal fluid (C.S.F.) to the incubation medium on the secretion of luteinizing hormone (LH) from adenohypophysial tissue, both in the presence and absence of hypothalami. Additionally, a study was made of the effect of including pineal bodies on the secretion of LH in both medium alone and in medium + C.S.F. No marked or consistent effect on LH secretion was observed when the C.S.F. concentration ranged between 5 per cent and 40 per cent, when the tissues were incubated in C.S.F. alone, nor when pineal bodies were added to the culture system. These results do not support the concept that the C.S.F. contains substantial amounts of substances which enhance secretion of LH from the anterior pituitary.

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