{"title":"基于树脂催化水解的尿羟脯氨酸自动分析。","authors":"S Orloff, V H Rao, L Verbruggen","doi":"10.1515/cclm.1975.13.12.549","DOIUrl":null,"url":null,"abstract":"<p><p>An automated method for the analysis of total urinary hydroxyproline using strong cation resin tablets of Hypronosticon is described for use in a clinical laboratory and the results are compared with those obtained by other methods. Even though good recoveries are obtained using the technique described in the present work by adding the internal standards either before or after hydrolysis of urine, the present method gave consistently lower values of urinary hydroxyproline compared with a manual and an automated method.</p>","PeriodicalId":23822,"journal":{"name":"Zeitschrift fur klinische Chemie und klinische Biochemie","volume":"13 12","pages":"549-52"},"PeriodicalIF":0.0000,"publicationDate":"1975-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/cclm.1975.13.12.549","citationCount":"0","resultStr":"{\"title\":\"Automated analysis of total urinary hydroxyproline based on resin-catalysed hydrolysis.\",\"authors\":\"S Orloff, V H Rao, L Verbruggen\",\"doi\":\"10.1515/cclm.1975.13.12.549\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>An automated method for the analysis of total urinary hydroxyproline using strong cation resin tablets of Hypronosticon is described for use in a clinical laboratory and the results are compared with those obtained by other methods. Even though good recoveries are obtained using the technique described in the present work by adding the internal standards either before or after hydrolysis of urine, the present method gave consistently lower values of urinary hydroxyproline compared with a manual and an automated method.</p>\",\"PeriodicalId\":23822,\"journal\":{\"name\":\"Zeitschrift fur klinische Chemie und klinische Biochemie\",\"volume\":\"13 12\",\"pages\":\"549-52\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1975-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1515/cclm.1975.13.12.549\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zeitschrift fur klinische Chemie und klinische Biochemie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/cclm.1975.13.12.549\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur klinische Chemie und klinische Biochemie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/cclm.1975.13.12.549","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Automated analysis of total urinary hydroxyproline based on resin-catalysed hydrolysis.
An automated method for the analysis of total urinary hydroxyproline using strong cation resin tablets of Hypronosticon is described for use in a clinical laboratory and the results are compared with those obtained by other methods. Even though good recoveries are obtained using the technique described in the present work by adding the internal standards either before or after hydrolysis of urine, the present method gave consistently lower values of urinary hydroxyproline compared with a manual and an automated method.
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