某些一年生草品种遗传异同的测定

Mahmut Kaplan, Nadide Kılınc, H. Pınar
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引用次数: 0

摘要

利用SRAP标记系统对25个一年生牧草品种进行遗传异同分析。平均多态性率为87%,其中Me4 Em6、Me4 Em7、Me2 Em13、Me4 Em4、Me4 Em9、Me4 Em11、Me4 Em12、Me2 Em14和Me3 Em2引物组合多态性最高。Me3 - Em3引物组合多态性最低(70%)。利用SRAP分子标记对禾草品种进行分子鉴定得到的树形图显示,不同基因型间存在较大差异。树状图的相似度在0.54 ~ 0.90之间。综上所述,SRAP标记体系是测定不同草品种间遗传异同的有效标记体系。此外,本研究中所获得的一年生草品种间的变异可用于牧草应用和育种计划。
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DETERMINATION OF THE GENETIC SIMILARITIES/DIFFERENCES OF SOME ANNUAL GRASS VARIETIES
This study was conducted to determine the genetic similarities/differences of twenty-five annual grass varieties using the SRAP marker system. While the mean polymorphism rate was determined as 87%, the highest polymorphism was determined in the Me4 Em6, Me4 Em7, Me2 Em13, Me4 Em4, Me4 Em9, Me4 Em11, Me4 Em12, Me2 Em14 and Me3 Em2 primer combinations. The lowest polymorphism rate was obtained from Me3 Em3 (70%) primer combination. According to the dendrogram obtained from the molecular characterization of grass varieties using SRAP molecular markers, great variation was observed between the genotypes. The degree of similarity in the dendrogram varied between 0.54-0.90. According to the findings, it can be said that the SRAP marker system was an effective marker system in determining the genetic similarities and differences between different grass varieties. In addition, the variation obtained among the annual grass varieties used in the study can be used both in the grass application and in breeding programs.
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