{"title":"小鼠脑细胞的悬浮摄氧量。","authors":"H Kovárů, Z Lodin","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A cell suspension was prepared by sieving mouse brain cortices in an isotonic solution of purified polyvinylpyrrolidone. A large increase on the O2-uptake by the suspension could be obtained with the preparation procedure described. The respiratory rate of suspension, incubated in saline medium, containing 6.2 mM K+, 10 mM pyruvate, 5 mM fumarate and 0.9 mM 5'-AMP, was equal to 68% of the respiratory rate of slices. High K+ concentration (65 mM) stimulated O2-consumption of suspension by 64% (73% in slices).</p>","PeriodicalId":19126,"journal":{"name":"Neurobiology","volume":"5 5","pages":"249-53"},"PeriodicalIF":0.0000,"publicationDate":"1975-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Oxygen uptake by suspension of mouse brain cells.\",\"authors\":\"H Kovárů, Z Lodin\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A cell suspension was prepared by sieving mouse brain cortices in an isotonic solution of purified polyvinylpyrrolidone. A large increase on the O2-uptake by the suspension could be obtained with the preparation procedure described. The respiratory rate of suspension, incubated in saline medium, containing 6.2 mM K+, 10 mM pyruvate, 5 mM fumarate and 0.9 mM 5'-AMP, was equal to 68% of the respiratory rate of slices. High K+ concentration (65 mM) stimulated O2-consumption of suspension by 64% (73% in slices).</p>\",\"PeriodicalId\":19126,\"journal\":{\"name\":\"Neurobiology\",\"volume\":\"5 5\",\"pages\":\"249-53\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1975-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Neurobiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurobiology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
用纯化的聚乙烯吡咯烷酮等渗溶液筛过小鼠脑皮层，制备细胞悬浮液。采用所述的制备方法，可以大大增加悬浮液对o2的吸收。在含6.2 mM K+、10 mM丙酮酸、5 mM富马酸和0.9 mM 5′-AMP的生理盐水培养基中培养悬液，呼吸率为切片呼吸率的68%。高K+浓度(65 mM)刺激悬浮液64%(切片73%)的臭氧消耗。
A cell suspension was prepared by sieving mouse brain cortices in an isotonic solution of purified polyvinylpyrrolidone. A large increase on the O2-uptake by the suspension could be obtained with the preparation procedure described. The respiratory rate of suspension, incubated in saline medium, containing 6.2 mM K+, 10 mM pyruvate, 5 mM fumarate and 0.9 mM 5'-AMP, was equal to 68% of the respiratory rate of slices. High K+ concentration (65 mM) stimulated O2-consumption of suspension by 64% (73% in slices).
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